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Structure models and predicted function of all proteins in JCVI-syn3.0

JCVI-syn3.0 accession and NCBI annotation	JCVI-syn3A accession and NCBI annotation	Our prediction (click to view)a	Closest homolog in M. mycoides (sequence identity)	TIGRFAMs class	Essentiality (b)
AMW76285 (MMSYN1_0001) DnaA: initiation of DNA replication in bacteria	AVX54569 (JCVISYN3A_0001) dnaA: Chromosomal replication initiator protein	GO:0003688 (C-score=0.65) DNA replication origin binding	C7MID5_MYCML (100.00%)	5 Equivalog	Essential
AMW76286 (MMSYN1_0002) DNA polymerase III, beta subunit: sliding clamp	AVX54570 (JCVISYN3A_0002) dnaN: DNA polymerase III subunit beta	GO:0003887 (C-score=0.85) DNA-directed DNA polymerase activity	C7LLN1_MYCML (100.00%)	5 Equivalog	Essential
AMW76287 (MMSYN1_0003) RnmV: processing of the 5' and 3' ends of 5S	AVX54571 (JCVISYN3A_0003) rnmV: Ribonuclease M5	GO:0043822 (C-score=1.00) ribonuclease M5 activity	C7LLN2_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76288 (MMSYN1_0004) KsgA: rRNA adenine dimethyltransferase	AVX54572 (JCVISYN3A_0004) ksgA: 16S rRNA (adenine(1518)- N(6)/adenine(1519)-N(6))-dimet hyltransferase	GO:0052908 (C-score=0.84) 16S rRNA (adenine(1518)-N(6)/a denine(1519)-N(6))-dimethyltra nsferase activity	C7LLN3_MYCML (100.00%)	5 Equivalog	Non essential
AMW76289 (MMSYN1_0005) hypothetical protein	AVX54573 (JCVISYN3A_0005) Uncharacterized protein	GO:0003779 (C-score=0.51) actin binding	C7LLN4_MYCML (100.00%)	1 Unknown	Non essential
AMW76290 (MMSYN1_0006) GyrB: catalyzes the interconversion of DNA	AVX54574 (JCVISYN3A_0006) gyrB: DNA gyrase subunit B	GO:0003918 (C-score=0.99) DNA topoisomerase type II (ATP-hydrolyzing) activity	C7LLN5_MYCML (100.00%)	5 Equivalog	Essential
AMW76291 (MMSYN1_0007) GyrA: catalyzes the interconversion of DNA	AVX54575 (JCVISYN3A_0007) gyrA: DNA gyrase subunit A	GO:0003918 (C-score=1.00) DNA topoisomerase type II (ATP-hydrolyzing) activity	C7LLN6_MYCML (100.00%)	5 Equivalog	Essential
AMW76292 (MMSYN1_0008) RnsD: nucleoside ABC transporter, permease	AVX54576 (JCVISYN3A_0008) rnsD;uptD: Nucleoside ABC transporter permease	GO:0015145 (C-score=0.83) monosaccharide transmembrane transporter activity	Q6MUM0_MYCMS (99.69%)	3 Putative	Essential
AMW76293 (MMSYN1_0009) RnsC: nucleoside ABC transporter, permease	AVX54577 (JCVISYN3A_0009) rnsC;uptD: Nucleoside ABC transporter permease	GO:0006139 (C-score=0.56) nucleobase-containing compound metabolic process	Q6MUL9_MYCMS (96.37%)	3 Putative	Essential
AMW76294 (MMSYN1_0010) RnsA: nucleoside ABC transporter, ATP-binding	AVX54578 (JCVISYN3A_0010) rnsA;uptA: Nucleoside ABC transporter ATP-binding protein	GO:0042626 (C-score=0.89) ATPase activity, coupled to transmembrane movement of substances	Q6MUL8_MYCMS (96.65%)	3 Putative	Essential
AMW76295 (MMSYN1_0011) RnsB: nucleoside ABC transporter, solute binding	AVX54579 (JCVISYN3A_0011) rnsB;uptB: Nucleoside ABC transporter substrate-binding protein	GO:0008643 (C-score=0.50) carbohydrate transport	Q6MUL7_MYCMS (96.90%)	3 Putative	Essential
AMW76296 (MMSYN1_0012) MetRS: Methionyl-tRNA synthetase	AVX54580 (JCVISYN3A_0012) metRS: Methionine--tRNA ligase	GO:0006431 (C-score=0.94) methionyl-tRNA aminoacylation	Q6MUL6_MYCMS (94.50%)	5 Equivalog	Essential
AMW76297 (MMSYN1_0025) S18: ribosomal protein S18	AVX54583 (JCVISYN3A_0025) rpsR: 30S ribosomal protein S18	GO:0006412 (C-score=0.99) translation	RS18_MYCMS (100.00%)	5 Equivalog	Essential
AMW76298 (MMSYN1_0026) SsbA: single-strand DNA- binding protein	AVX54584 (JCVISYN3A_0026) priB: Single-stranded DNA- binding protein	GO:0051096 (C-score=0.54) positive regulation of helicase activity	Q6MUK5_MYCMS (97.95%)	4 Probable	Essential
AMW76299 (MMSYN1_0027) S6: ribosomal protein S6	AVX54585 (JCVISYN3A_0027) rpsF: 30S ribosomal protein S6	GO:0010467 (C-score=0.51) gene expression	RS6_MYCMS (99.27%)	5 Equivalog	Essential
AMW76300 (MMSYN1_0029) FMN-dependent NADH- azoreductase 1 family	AVX54586 (JCVISYN3A_0029) Uncharacterized FMN-dependent NADH-azoreductase	GO:0008752 (C-score=0.85) FMN reductase activity	C7LLQ7_MYCML (100.00%)	2 Generic	Essential
AMW76301 (MMSYN1_0030) ABC transporter, ATP-binding protein	AVX54587 (JCVISYN3A_0030) Uncharacterized ABC transporter ATP-binding protein	GO:0006869 (C-score=0.58) lipid transport	Q6MUK1_MYCMS (97.38%)	2 Generic	Essential
AMW76302 (MMSYN1_0033) hypothetical protein	AVX54588 (JCVISYN3A_0033) Uncharacterized protein	GO:0016926 (C-score=0.60) protein desumoylation	Q6MUJ9_MYCMS (64.22%)	1 Unknown	Essential
AMW76303 (MMSYN1_0034) efflux ABC transporter, permease protein	AVX54589 (JCVISYN3A_0034) Uncharacterized efflux ABC transporter permease	GO:0010467 (C-score=0.58) gene expression	C7LLR2_MYCML (99.83%)	2 Generic	Non essential
AMW76304 (MMSYN1_0039) Putative FtsH peptidase	AVX54590 (JCVISYN3A_0039) ftsH: Membrane anchored AAA+ protease	GO:0030554 (C-score=0.55) adenyl nucleotide binding	Q6MUJ2_MYCMS (97.53%)	2 Generic	Essential
AMW76305 (MMSYN1_0040) TilS: tRNA(Ile)-lysidine synthase	AVX54591 (JCVISYN3A_0040) tilS: tRNA lysidine(34) synthetase	GO:0034227 (C-score=0.65) tRNA thio-modification	TILS_MYCMS (94.76%)	5 Equivalog	Essential
AMW76306 (MMSYN1_0042) transcriptional regulator, RpiR family	AVX54592 (JCVISYN3A_0042) Uncharacterized transcriptional regulator	GO:0004360 (C-score=0.79) glutamine-fructose-6-phosphate transaminase (isomerizing) activity	Q6MUI8_MYCMS (96.36%)	2 Generic	Quasi essential
AMW76307 (MMSYN1_0043) ribosomal protein L11 methyltransferase-like	AVX54593 (JCVISYN3A_0043) yabD: Uncharacterized methyltransferase	GO:0008170 (C-score=0.80) N-methyltransferase activity	Q6MUI7_MYCMS (97.50%)	2 Generic	Non essential
AMW76308 (MMSYN1_0044) DNA polymerase III delta subunit	AVX54594 (JCVISYN3A_0044) holA: DNA polymerase III subunit delta	GO:0006272 (C-score=0.50) leading strand elongation	Q6MUI6_MYCMS (96.34%)	4 Probable	Essential
AMW76309 (MMSYN1_0045) tmk: thymidylate kinase	AVX54595 (JCVISYN3A_0045) tmk: dTMP kinase	GO:0046940 (C-score=0.88) nucleoside monophosphate phosphorylation	KTHY_MYCMS (96.71%)	5 Equivalog	Essential
AMW76310 (MMSYN1_0046) RecR: Recombination protein	AVX54596 (JCVISYN3A_0046) recR: Recombination protein	GO:0006302 (C-score=0.56) double-strand break repair	Q6MUI4_MYCMS (69.81%)	5 Equivalog	Quasi essential
AMW76311 (MMSYN1_0047) DNA polymerase III, subunit gamma and tau	AVX54597 (JCVISYN3A_0047) dnaX: DNA polymerase III subunit gamma and tau	GO:0003689 (C-score=0.97) DNA clamp loader activity	Q6MUI3_MYCMS (95.46%)	5 Equivalog	Essential
AMW76312 (MMSYN1_0054) Putative redoxin	AVX54598 (JCVISYN3A_0054) ahpC: Uncharacterized peroxiredoxin	GO:0008379 (C-score=0.56) thioredoxin peroxidase activity	Q6MUH8_MYCMS (97.32%)	2 Generic	Non essential
AMW76313 (MMSYN1_0060) membrane protein, putative	AVX54599 (JCVISYN3A_0060) Uncharacterized protein	GO:0006418 (C-score=0.50) tRNA aminoacylation for protein translation	C7LLT6_MYCML (100.00%)	1 Unknown	Quasi essential
AMW76314 (MMSYN1_0061) SerRS: Seryl-tRNA synthetase	AVX54600 (JCVISYN3A_0061) serRS: Serine--tRNA ligase	GO:0016260 (C-score=0.57) selenocysteine biosynthetic process	C7LLT7_MYCML (100.00%)	5 Equivalog	Essential
AMW76315 (MMSYN1_0063) putative tRNA-dihydrouridine synthase B	AVX54601 (JCVISYN3A_0063) dusB: Uncharacterized tRNA dihydrouridine synthase	GO:0002943 (C-score=0.80) tRNA dihydrouridine synthesis	C7LLT9_MYCML (100.00%)	2 Generic	Non essential
AMW76316 (MMSYN1_0064) LysRS: Lysyl-tRNA synthetase	AVX54602 (JCVISYN3A_0064) lysRS: Lysine--tRNA ligase	GO:0006430 (C-score=0.84) lysyl-tRNA aminoacylation	C7LLU0_MYCML (100.00%)	5 Equivalog	Essential
AMW76317 (MMSYN1_0065) TrxA: thioredoxin	AVX54603 (JCVISYN3A_0065) trxA: Thioredoxin	GO:0006662 (C-score=0.89) glycerol ether metabolic process	Q6MUG0_MYCMS (98.04%)	5 Equivalog	Essential
AMW76318 (MMSYN1_0066) Cof-like hydrolase	AVX54604 (JCVISYN3A_0066) Low specificity hydrolase	GO:0016311 (C-score=0.66) dephosphorylation	Q6MUF9_MYCMS (99.29%)	2 Generic	Quasi essential
AMW76319 (MMSYN1_0076) AsnRS: Asparaginyl-tRNA synthetase	AVX54605 (JCVISYN3A_0076) asnRS: Asparagine--tRNA ligase	GO:0006421 (C-score=0.53) asparaginyl-tRNA aminoacylation	SYN_MYCMS (98.23%)	5 Equivalog	Essential
AMW76320 (MMSYN1_0077) Cof-like hydrolase	AVX54606 (JCVISYN3A_0077) Uncharacterized hydrolase	GO:0016311 (C-score=0.71) dephosphorylation	Q6MUF1_MYCMS (97.13%)	2 Generic	Quasi essential
AMW76321 (MMSYN1_0079) TsaD: tRNA(NNU) t(6)A37	AVX54607 (JCVISYN3A_0079) tsaD: tRNA (adenosine(37)-N6)- threonylcarbamoyltransferase complex transferase subunit D	GO:0061711 (C-score=0.77) N(6)-L-threonylcarbamoyladenin e synthase	Q6MUE9_MYCMS (97.96%)	5 Equivalog	Essential
AMW76322 (MMSYN1_0080) PF09954 family protein	AVX54608 (JCVISYN3A_0080) Uncharacterized protein	GO:0016787 (C-score=0.66) hydrolase activity	Q6MUE8_MYCMS (96.60%)	1 Unknown	Non essential
AMW76323 (MMSYN1_0081) mnmE_trmE_thdF: tRNA modification GTPase TrmE	AVX54609 (JCVISYN3A_0081) mnmE;trmE: tRNA uridine(34) 5-carboxymethylaminomethyl synthesis GTPase	GO:0002098 (C-score=0.82) tRNA wobble uridine modification	C7LLV2_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76324 (MMSYN1_0082) S20: ribosomal protein S20	AVX54610 (JCVISYN3A_0082) rpsT: 30S ribosomal protein S20	GO:0008073 (C-score=0.53) ornithine decarboxylase inhibitor activity	RS20_MYCMS (100.00%)	5 Equivalog	Quasi essential
AMW76325 (MMSYN1_0094) membrane protein, putative	AVX54611 (JCVISYN3A_0094) Uncharacterized protein	GO:0005971 (C-score=0.92) ribonucleoside-diphosphate reductase complex	Q6MUE4_MYCMS (87.58%)	2 Generic	Non essential
AMW76326 (MMSYN1_0095) secA: preprotein translocase, SecA subunit	AVX54612 (JCVISYN3A_0095) secA: Preprotein translocase subunit A	GO:0043952 (C-score=0.74) protein transport by the Sec complex	SECA_MYCMS (98.20%)	5 Equivalog	Essential
AMW76327 (MMSYN1_0097) 5'-3' exonuclease, N-terminal resolvase-like	AVX54613 (JCVISYN3A_0097) yycJ: Uncharacterized 5'-3' exonuclease	GO:0017108 (C-score=0.62) 5'-flap endonuclease activity	C7LLW8_MYCML (100.00%)	2 Generic	Quasi essential
AMW76328 (MMSYN1_0105) XseB: exodeoxyribonuclease VII, small subunit	AVX54614 (JCVISYN3A_0105) xseB: Exodeoxyribonuclease VII small subunit	GO:0008855 (C-score=0.85) exodeoxyribonuclease VII activity	Q6MUD2_MYCMS (97.18%)	4 Probable	Non essential
AMW76329 (MMSYN1_0106) XseA, exodeoxyribonuclease VII, large subunit	AVX54615 (JCVISYN3A_0106) xseA: Exodeoxyribonuclease VII large subunit	GO:0008855 (C-score=0.51) exodeoxyribonuclease VII activity	EX7L_MYCMS (94.88%)	5 Equivalog	Non essential
AMW76330 (MMSYN1_0107) NusB: transcription antitermination factor NusB	AVX54616 (JCVISYN3A_0107) nusB: Transcription antitermination factor	GO:0031564 (C-score=0.61) transcription antitermination	Q6MUD0_MYCMS (93.94%)	5 Equivalog	Quasi essential
AMW76331 (MMSYN1_0108) lipoprotein, putative	AVX54617 (JCVISYN3A_0108) Uncharacterized lipoprotein	GO:0008144 (C-score=0.56) drug binding	Q6MUC9_MYCMS (81.69%)	2 Generic	Quasi essential
AMW76332 (MMSYN1_0109) Putative apurinic endonuclease	AVX54618 (JCVISYN3A_0109) Deoxyribonuclease IV	GO:0003906 (C-score=0.99) DNA-(apurinic or apyrimidinic site) lyase activity	END4_MYCMS (96.54%)	2 Generic	Quasi essential
AMW76333 (MMSYN1_0113) glycosyltransferase, group 2 family protein	AVX54619 (JCVISYN3A_0113) bcsA: Glycolipid synthase A	GO:0033692 (C-score=0.61) cellular polysaccharide biosynthetic process	C7LLY3_MYCML (100.00%)	3 Putative	Essential
AMW76334 (MMSYN1_0114) glycosyltransferase, group 2 family protein	AVX54620 (JCVISYN3A_0114) bcsB;cps: Glycolipid synthase B	GO:0019348 (C-score=0.63) dolichol metabolic process	Q6MRZ4_MYCMS (93.11%)	3 Putative	Essential
AMW76335 (MMSYN1_0115) galU: UTP--glucose-1-phosphate	AVX54621 (JCVISYN3A_0115) galU;gtaB: UTP-- glucose-1-phosphate uridylyltransferase	GO:0003983 (C-score=0.95) UTP:glucose-1-phosphate uridylyltransferase activity	Q6MUC2_MYCMS (93.79%)	4 Probable	Essential
AMW76336 (MMSYN1_0116) hypothetical protein	AVX54622 (JCVISYN3A_0116) Uncharacterized protein	GO:0055086 (C-score=0.54) nucleobase-containing small molecule metabolic process	Q6MUC1_MYCMS (94.52%)	1 Unknown	Quasi essential
AMW76337 (MMSYN1_0117) plsY: glycerol-3-phosphate acyltransferase	AVX54623 (JCVISYN3A_0117) plsY: Acyl-phosphate glycerol 3-phosphate acyltransferase	GO:0005215 (C-score=0.51) transporter activity	C7LLY7_MYCML (100.00%)	5 Equivalog	Essential
AMW76338 (MMSYN1_0126) GluRS: Glutamyl-tRNA synthetase	AVX54624 (JCVISYN3A_0126) gluRS: Glutamate--tRNA ligase	GO:0006424 (C-score=0.96) glutamyl-tRNA aminoacylation	C7LLZ6_MYCML (100.00%)	5 Equivalog	Essential
AMW76339 (MMSYN1_0127) HD domain protein	AVX54625 (JCVISYN3A_0127) Uncharacterized phosphohydrolase	GO:0008832 (C-score=1.00) dGTPase activity	C7LLZ7_MYCML (100.00%)	2 Generic	Non essential
AMW76340 (MMSYN1_0128) RNA polymerase delta subunit	AVX54626 (JCVISYN3A_0128) rpoE: DNA-directed RNA polymerase subunit delta	GO:0000448 (C-score=0.92) cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU- rRNA)	C7LLZ8_MYCML (100.00%)	4 Probable	Quasi essential
AMW76341 (MMSYN1_0129) PyrG: CTP synthase	AVX54627 (JCVISYN3A_0129) pyrG: CTP synthase	GO:0044210 (C-score=0.89) 'de novo' CTP biosynthetic process	C7LLZ9_MYCML (100.00%)	5 Equivalog	Essential
AMW76342 (MMSYN1_0131) fruc_bis_ald_: fructose-1,6-bisphosphate	AVX54628 (JCVISYN3A_0131) fbaA: Fructose-1,6-bisphosphate aldolase	GO:0030388 (C-score=0.86) fructose 1,6-bisphosphate metabolic process	Q6MU98_MYCMS (100.00%)	5 Equivalog	Essential
AMW76343 (MMSYN1_0132) ATPase, AAA family	AVX54629 (JCVISYN3A_0132) ietA: Toxin-antitoxin AAA ATPase	GO:0045898 (C-score=0.62) regulation of RNA polymerase II transcriptional preinitiation complex assembly	C7LM02_MYCML (100.00%)	2 Generic	Essential
AMW76344 (MMSYN1_0133) peptidase, S8/S53 family	AVX54630 (JCVISYN3A_0133) ietS: Toxin-antitoxin serine protease	GO:0004252 (C-score=0.98) serine-type endopeptidase activity	C7LM03_MYCML (100.00%)	2 Generic	Non essential
AMW76345 (MMSYN1_0137) L31: ribosomal protein L31	AVX54631 (JCVISYN3A_0137) rpmE: 50S ribosomal protein L31	GO:0006412 (C-score=0.69) translation	C7LM07_MYCML (100.00%)	5 Equivalog	Essential
AMW76346 (MMSYN1_0138) hypothetical protein	AVX54632 (JCVISYN3A_0138) Uncharacterized protein	GO:0032561 (C-score=0.53) guanyl ribonucleotide binding	C7LM08_MYCML (100.00%)	1 Unknown	Quasi essential
AMW76347 (MMSYN1_0139) DHHA1 domain protein	AVX54633 (JCVISYN3A_0139) ytqI;orn: NanoRNAse	GO:0004427 (C-score=0.91) inorganic diphosphatase activity	C7LM09_MYCML (100.00%)	2 Generic	Quasi essential
AMW76348 (MMSYN1_0140) tdk: deoxythymidine kinase	AVX54634 (JCVISYN3A_0140) tdk: Thymidine kinase	GO:0004797 (C-score=0.99) thymidine kinase activity	C7LM10_MYCML (100.00%)	4 Probable	Essential
AMW76349 (MMSYN1_0141) PrfA: peptide chain release factor 1	AVX54635 (JCVISYN3A_0141) prfA: Peptide chain release factor 1	GO:0016149 (C-score=0.99) translation release factor activity, codon specific	C7LM11_MYCML (100.00%)	5 Equivalog	Essential
AMW76350 (MMSYN1_0142) PrmC: Release factor glutamine	AVX54636 (JCVISYN3A_0142) prmC: Protein-(glutamine-N5) methyltransferase, release factor-specific	GO:0036009 (C-score=0.82) protein-glutamine N-methyltransferase activity	C7LM12_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76351 (MMSYN1_0143) membrane protein, putative	AVX54637 (JCVISYN3A_0143) Uncharacterized protein	GO:0016740 (C-score=0.53) transferase activity	C7LM13_MYCML (100.00%)	1 Unknown	Essential
AMW76352 (MMSYN1_0144) tsaC: tRNA(ANN) t(6)A37	AVX54638 (JCVISYN3A_0144) tsaC: tRNA (adenosine(37)-N6)- threonylcarbamoyltransferase complex transferase subunit	GO:0002949 (C-score=0.96) tRNA threonylcarbamoyladenosine modification	C7LM14_MYCML (100.00%)	4 Probable	Essential
AMW76353 (MMSYN1_0145) Acetyltransferase, GNAT family	AVX54639 (JCVISYN3A_0145) Uncharacterized acetyltransferase	GO:0004596 (C-score=0.90) peptide alpha-N-acetyltransferase activity	C7LM15_MYCML (100.00%)	2 Generic	Essential
AMW76354 (MMSYN1_0146) hypothetical protein	AVX54640 (JCVISYN3A_0146) Uncharacterized protein	GO:0005576 (C-score=0.89) extracellular region	C7LM16_MYCML (100.00%)	1 Unknown	Essential
AMW76355 (MMSYN1_0147) bac_cardiolipin: cardiolipin synthase	AVX54641 (JCVISYN3A_0147) clsA: Cardiolipin synthase	GO:0008808 (C-score=0.91) cardiolipin synthase activity	C7LM17_MYCML (100.00%)	5 Equivalog	Essential
AMW76356 (MMSYN1_0148) S12: ribosomal protein S12	AVX54642 (JCVISYN3A_0148) rpsL: 30S ribosomal protein S12	GO:0006412 (C-score=0.99) translation	RS12_MYCMS (100.00%)	5 Equivalog	Essential
AMW76357 (MMSYN1_0149) S7: ribosomal protein S7	AVX54643 (JCVISYN3A_0149) rpsG: 30S ribosomal protein S7	GO:0000028 (C-score=0.99) ribosomal small subunit assembly	C7LM19_MYCML (99.35%)	5 Equivalog	Essential
AMW76358 (MMSYN1_0150) EF-G: translation elongation factor G	AVX54644 (JCVISYN3A_0150) Translation elongation factor G	GO:0006414 (C-score=0.72) translational elongation	C7LM20_MYCML (100.00%)	5 Equivalog	Essential
AMW76359 (MMSYN1_0151) EF-Tu: translation elongation factor Tu	AVX54645 (JCVISYN3A_0151) Translation elongation factor Tu	GO:0006414 (C-score=0.68) translational elongation	EFTU_MYCMS (100.00%)	5 Equivalog	Essential
AMW76360 (MMSYN1_0163) AlaRS: Alanyl-tRNA synthetase	AVX54646 (JCVISYN3A_0163) alaRS: Alanine--tRNA ligase	GO:0006419 (C-score=0.92) alanyl-tRNA aminoacylation	SYA_MYCMS (97.32%)	5 Equivalog	Essential
AMW76361 (MMSYN1_0164) hypothetical protein	AVX54647 (JCVISYN3A_0164) Uncharacterized protein	GO:0048037 (C-score=0.58) cofactor binding	Q6MU62_MYCMS (95.12%)	1 Unknown	Quasi essential
AMW76362 (MMSYN1_0165) Putative AmiC	AVX54648 (JCVISYN3A_0165) amiC: Oligopeptide ABC transporter permease	GO:0042626 (C-score=0.64) ATPase activity, coupled to transmembrane movement of substances	C7LM32_MYCML (100.00%)	2 Generic	Quasi essential
AMW76363 (MMSYN1_0166) Putative AmiD	AVX54649 (JCVISYN3A_0166) Oligopeptide ABC transporter permease	GO:0042626 (C-score=0.50) ATPase activity, coupled to transmembrane movement of substances	C7LM33_MYCML (100.00%)	2 Generic	Quasi essential
AMW76364 (MMSYN1_0167) Putative AmiE	AVX54650 (JCVISYN3A_0167) amiE: Oligopeptide ABC transporter ATP-binding protein	GO:0032559 (C-score=0.50) adenyl ribonucleotide binding	C7LM34_MYCML (100.00%)	2 Generic	Quasi essential
AMW76365 (MMSYN1_0168) Putative AmiF	AVX54651 (JCVISYN3A_0168) amiF: Oligopeptide ABC transporter ATP-binding protein	GO:0035639 (C-score=0.59) purine ribonucleoside triphosphate binding	C7LM35_MYCML (100.00%)	2 Generic	Quasi essential
AMW76366 (MMSYN1_0169) Putative AmiA	AVX54652 (JCVISYN3A_0169) amiA: Oligopeptide ABC transporter substrate-binding protein	GO:0006857 (C-score=0.68) oligopeptide transport	C7LM36_MYCML (100.00%)	2 Generic	Quasi essential
AMW76367 (MMSYN1_0195) Putative potCD or potHI	AVX54653 (JCVISYN3A_0195) potC: Spermidine/putrescine ABC transporter permease	GO:0022891 (C-score=0.60) substrate-specific transmembrane transporter activity	Q6MU17_MYCMS (94.22%)	2 Generic	Quasi essential
AMW76368 (MMSYN1_0196) Putative potB or potG	AVX54654 (JCVISYN3A_0196) potB: Spermidine/putrescine ABC transporter permease	GO:0006820 (C-score=0.53) anion transport	Q6MU18_MYCMS (97.88%)	2 Generic	Quasi essential
AMW76369 (MMSYN1_0197) Putative potA or potF	AVX54655 (JCVISYN3A_0197) potA: Spermidine/putrescine ABC transporter ATP-binding protein	GO:0035639 (C-score=0.94) purine ribonucleoside triphosphate binding	POTA_MYCMS (99.15%)	2 Generic	Quasi essential
AMW76370 (MMSYN1_0198) L20: ribosomal protein L20	AVX54656 (JCVISYN3A_0198) rplT: 50S ribosomal protein L20	GO:0000027 (C-score=1.00) ribosomal large subunit assembly	RL20_MYCMS (96.67%)	5 Equivalog	Essential
AMW76371 (MMSYN1_0199) L35: ribosomal protein L35	AVX54657 (JCVISYN3A_0199) rpmI: 50S ribosomal protein L35	GO:0006412 (C-score=0.75) translation	RL35_MYCMS (100.00%)	5 Equivalog	Essential
AMW76372 (MMSYN1_0200) infC: translation initiation factor IF-3	AVX54658 (JCVISYN3A_0200) infC: Translation initiation factor IF-3	GO:0032790 (C-score=1.00) ribosome disassembly	IF3_MYCMS (100.00%)	5 Equivalog	Essential
AMW76373 (MMSYN1_0201) pept_deformyl: peptide deformylase	AVX54659 (JCVISYN3A_0201) def: Peptide deformylase	GO:0042586 (C-score=1.00) peptide deformylase activity	Q6MU23_MYCMS (97.00%)	5 Equivalog	Essential
AMW76374 (MMSYN1_0202) RsmD: 16S rRNA	AVX54660 (JCVISYN3A_0202) rsmD: 16S rRNA (guanine(966)-N (2))-methyltransferase	GO:0052913 (C-score=0.73) 16S rRNA (guanine(966)-N(2))-m ethyltransferase activity	C7LM67_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76375 (MMSYN1_0203) guanyl_kin: guanylate kinase	AVX54661 (JCVISYN3A_0203) gmk: Guanylate kinase	GO:0035255 (C-score=0.51) ionotropic glutamate receptor binding	C7LM68_MYCML (100.00%)	5 Equivalog	Essential
AMW76376 (MMSYN1_0213) eno: phosphopyruvate hydratase	AVX54662 (JCVISYN3A_0213) eno: Phosphopyruvate hydratase	GO:0004634 (C-score=0.97) phosphopyruvate hydratase activity	C7LM77_MYCML (99.78%)	5 Equivalog	Essential
AMW76377 (MMSYN1_0214) phosphatidylglycerophosphatase	AVX54663 (JCVISYN3A_0214) pgpA: Phosphatidylglycerophosphatase	GO:0046839 (C-score=0.83) phospholipid dephosphorylation	C7LM78_MYCML (100.00%)	3 Putative	Quasi essential
AMW76378 (MMSYN1_0215) RNAse H domain protein, YqgF family	AVX54664 (JCVISYN3A_0215) yrrK;yqgF: Putative pre-16S rRNA nuclease	GO:0031564 (C-score=0.61) transcription antitermination	C7LM79_MYCML (100.00%)	2 Generic	Essential
AMW76379 (MMSYN1_0216) HGPRTase: hypoxanthine	AVX54665 (JCVISYN3A_0216) hptA: Hypoxanthine phosphoribosyltransferase	GO:0046038 (C-score=0.53) GMP catabolic process	C7LM80_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76380 (MMSYN1_0218) glycerol_kin: glycerol kinase	AVX54666 (JCVISYN3A_0218) glpK: Glycerol kinase	GO:0004370 (C-score=0.89) glycerol kinase activity	GLPK_MYCMS (98.61%)	5 Equivalog	Essential
AMW76381 (MMSYN1_0220) PFKA_ATP: 6-phosphofructokinase	AVX54667 (JCVISYN3A_0220) pfkA: 6-Phosphofructokinase	GO:0070095 (C-score=0.70) fructose-6-phosphate binding	C7LM84_MYCML (100.00%)	5 Equivalog	Essential
AMW76382 (MMSYN1_0221) pyruv_kin: pyruvate kinase	AVX54668 (JCVISYN3A_0221) pyk: Pyruvate kinase	GO:0004743 (C-score=0.99) pyruvate kinase activity	C7LM85_MYCML (100.00%)	5 Equivalog	Essential
AMW76383 (MMSYN1_0222) ThrRS: Threonyl-tRNA synthetase	AVX54669 (JCVISYN3A_0222) thrRS: Threonine--tRNA ligase	GO:0006435 (C-score=0.83) threonyl-tRNA aminoacylation	C7LM86_MYCML (100.00%)	5 Equivalog	Essential
AMW76384 (MMSYN1_0227) PdhC: pyruvate dehydrogenase (dihydrolipoamide	AVX54670 (JCVISYN3A_0227) pdhC: Branched-chain alpha- keto acid dehydrogenase subunit E2	GO:0016417 (C-score=0.59) S-acyltransferase activity	C7LM91_MYCML (100.00%)	4 Probable	Non essential
AMW76385 (MMSYN1_0228) PdhD: lipoamide_DH: dihydrolipoyl dehydrogenase	AVX54671 (JCVISYN3A_0228) lpdA: Dihydrolipoyl dehydrogenase	GO:0004148 (C-score=0.93) dihydrolipoyl dehydrogenase activity	C7MJ02_MYCML (100.00%)	5 Equivalog	Non essential
AMW76386 (MMSYN1_0229) pta: phosphate acetyltransferase	AVX54672 (JCVISYN3A_0229) pta: Phosphate acetyltransferase	GO:0008959 (C-score=0.91) phosphate acetyltransferase activity	C7LM93_MYCML (100.00%)	5 Equivalog	Essential
AMW76387 (MMSYN1_0230) AckA: acetate kinase	AVX54673 (JCVISYN3A_0230) ackA: Acetate kinase	GO:0008980 (C-score=0.70) propionate kinase activity	C7LM94_MYCML (100.00%)	5 Equivalog	Essential
AMW76388 (MMSYN1_0233) PtsI: phosphoenolpyruvate- protein	AVX54674 (JCVISYN3A_0233) ptsI: Phosphoenolpyruvate-- protein phosphotransferase	GO:0008965 (C-score=0.63) phosphoenolpyruvate-protein phosphotransferase activity	C7LM97_MYCML (100.00%)	4 Probable	Essential
AMW76389 (MMSYN1_0234) crr: glucose-specific phosphotransferase enzyme	AVX54675 (JCVISYN3A_0234) crr: PTS glucose transporter subunit IIA	GO:0043610 (C-score=0.89) regulation of carbohydrate utilization	C7LM98_MYCML (100.00%)	4 Probable	Essential
AMW76390 (MMSYN1_0235) hypothetical protein	AVX54676 (JCVISYN3A_0235) Uncharacterized protein	GO:0043231 (C-score=0.50) intracellular membrane-bounded organelle	C7LM99_MYCML (100.00%)	1 Unknown	Quasi essential
AMW76391 (MMSYN1_0238) S4: ribosomal protein S4	AVX54677 (JCVISYN3A_0238) rpsD: 30S ribosomal protein S4	GO:0045903 (C-score=1.00) positive regulation of translational fidelity	RS4_MYCMS (100.00%)	5 Equivalog	Essential
AMW76392 (MMSYN1_0239) hypothetical protein	AVX54678 (JCVISYN3A_0239) Uncharacterized protein	GO:0007010 (C-score=0.50) cytoskeleton organization	C7LMA3_MYCML (100.00%)	1 Unknown	Non essential
AMW76393 (MMSYN1_0240) ThiI: Probable tRNA sulfurtransferase, ThiS	AVX54679 (JCVISYN3A_0240) thiI: tRNA 4-thiouridine(8) synthase	GO:0004810 (C-score=0.63) tRNA adenylyltransferase activity	C7LMA4_MYCML (100.00%)	4 Probable	Quasi essential
AMW76394 (MMSYN1_0247) GTPase_YsxC: ribosome biogenesis GTP-binding	AVX54680 (JCVISYN3A_0247) ysxC;yihA: Ribosome biogenesis GTP-binding protein	GO:0000917 (C-score=0.94) barrier septum assembly	ENGB_MYCMS (96.43%)	5 Equivalog	Essential
AMW76395 (MMSYN1_0248) hypothetical protein	AVX54681 (JCVISYN3A_0248) Uncharacterized protein	GO:0015318 (C-score=0.50) inorganic solute uptake transmembrane transporter activity	Q6MTV4_MYCMS (96.24%)	1 Unknown	Non essential
AMW76396 (MMSYN1_0249) hypothetical protein	AVX54682 (JCVISYN3A_0249) Uncharacterized protein	GO:0015318 (C-score=0.51) inorganic solute uptake transmembrane transporter activity	Q6MTV3_MYCMS (97.92%)	1 Unknown	Essential
AMW76397 (MMSYN1_0250) hypothetical protein	AVX54683 (JCVISYN3A_0250) Uncharacterized protein	GO:0015318 (C-score=0.50) inorganic solute uptake transmembrane transporter activity	Q6MTV2_MYCMS (96.51%)	1 Unknown	Non essential
AMW76398 (MMSYN1_0253) GreA: Transcription elongation factor	AVX54684 (JCVISYN3A_0253) greA: Transcription elongation factor	GO:0001108 (C-score=0.93) bacterial-type RNA polymerase holo enzyme binding	GREA_MYCMS (98.73%)	4 Probable	Essential
AMW76399 (MMSYN1_0254) uvrC: excinuclease ABC subunit C	AVX54685 (JCVISYN3A_0254) uvrC: Excinuclease ABC subunit C	GO:0006974 (C-score=0.60) cellular response to DNA damage stimulus	UVRC_MYCMS (98.12%)	5 Equivalog	Quasi essential
AMW76400 (MMSYN1_0257) RnjB: Ribonuclease J2	AVX54686 (JCVISYN3A_0257) rnjB: RNase J family beta-CASP ribonuclease	GO:0004534 (C-score=0.74) 5'-3' exoribonuclease activity	Q6MTU5_MYCMS (99.31%)	4 Probable	Essential
AMW76401 (MMSYN1_0259) NadK: NAD kinase	AVX54687 (JCVISYN3A_0259) nadK: NAD(+) kinase	GO:0006741 (C-score=0.99) NADP biosynthetic process	Q6MTU3_MYCMS (99.25%)	3 Putative	Essential
AMW76402 (MMSYN1_0260) ValRS: Valyl-tRNA synthetase	AVX54688 (JCVISYN3A_0260) valRS: Valine--tRNA ligase	GO:0006438 (C-score=0.60) valyl-tRNA aminoacylation	SYV_MYCMS (97.48%)	5 Equivalog	Essential
AMW76403 (MMSYN1_0262) ribulose-phosphate 3-epimerase	AVX54689 (JCVISYN3A_0262) rpe: Ribulose-phosphate 3-epimerase	GO:0004750 (C-score=1.00) ribulose-phosphate 3-epimerase activity	Q6MTU0_MYCMS (96.00%)	5 Equivalog	Quasi essential
AMW76404 (MMSYN1_0263) RsgA: ribosome small subunit- dependent GTPase A	AVX54690 (JCVISYN3A_0263) Ribosome small subunit- dependent GTPase A	GO:0042274 (C-score=0.88) ribosomal small subunit biogenesis	Q6MTT9_MYCMS (95.33%)	5 Equivalog	Quasi essential
AMW76405 (MMSYN1_0264) kinase domain protein	AVX54691 (JCVISYN3A_0264) Uncharacterized serine/threonine protein kinase	GO:0006915 (C-score=0.60) apoptotic process	Q6MTT8_MYCMS (95.96%)	2 Generic	Quasi essential
AMW76406 (MMSYN1_0270) YjeE: tRNA threonylcarbamoyl adenosine	AVX54692 (JCVISYN3A_0270) tsaE;yjeE: tRNA (N6-adenosine( 37)-N6)-threonylcarbamoyltrans ferase complex ATPase	GO:0002949 (C-score=0.86) tRNA threonylcarbamoyladenosine modification	C7LMD2_MYCML (100.00%)	5 Equivalog	Essential
AMW76407 (MMSYN1_0271) YeaZ: tRNA threonylcarbamoyl adenosine	AVX54693 (JCVISYN3A_0271) tsaB;yeaZ: tRNA N6-adenosine(3 7)-N6-threonylcarbamoyltransfe rase complex dimerization subunit	GO:0002949 (C-score=0.99) tRNA threonylcarbamoyladenosine modification	C7LMD3_MYCML (100.00%)	5 Equivalog	Essential
AMW76408 (MMSYN1_0281) hypothetical protein	AVX54694 (JCVISYN3A_0281) Uncharacterized protein	GO:0016788 (C-score=0.50) hydrolase activity, acting on ester bonds	C7LME1_MYCML (100.00%)	1 Unknown	Essential
AMW76409 (MMSYN1_0282) ProRS: Prolyl-tRNA synthetase	AVX54695 (JCVISYN3A_0282) proRS: Proline--tRNA ligase	GO:0006433 (C-score=0.96) prolyl-tRNA aminoacylation	C7LME2_MYCML (100.00%)	5 Equivalog	Essential
AMW76410 (MMSYN1_0283) caulimovirus viroplasmin / ribonuclease HI	AVX54696 (JCVISYN3A_0283) rnhA: Double-stranded RNA binding RNase HI	GO:0043137 (C-score=0.96) DNA replication, removal of RNA primer	C7LME3_MYCML (100.00%)	2 Generic	Essential
AMW76411 (MMSYN1_0285) lepA: elongation factor 4	AVX54697 (JCVISYN3A_0285) lepA;EF4: Elongation factor 4	GO:0006414 (C-score=0.74) translational elongation	C7LME5_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76412 (MMSYN1_0286) hypothetical protein	AVX54698 (JCVISYN3A_0286) Uncharacterized protein	GO:1901363 (C-score=0.57) heterocyclic compound binding	Q6MTR5_MYCMS (92.22%)	1 Unknown	Essential
AMW76413 (MMSYN1_0287) AspRS: Aspartyl-tRNA synthetase	AVX54699 (JCVISYN3A_0287) aspRS: Aspartate--tRNA ligase	GO:0006422 (C-score=0.65) aspartyl-tRNA aminoacylation	SYD_MYCMS (97.21%)	5 Equivalog	Essential
AMW76414 (MMSYN1_0288) HisRS: Histidyl-tRNA synthetase	AVX54700 (JCVISYN3A_0288) hisRS: Histidine--tRNA ligase	GO:0006427 (C-score=0.97) histidyl-tRNA aminoacylation	SYH_MYCMS (99.28%)	5 Equivalog	Essential
AMW76415 (MMSYN1_0289) RbfA: ribosome-binding factor A	AVX54701 (JCVISYN3A_0289) rbfA: Ribosome-binding factor A	GO:0043024 (C-score=0.69) ribosomal small subunit binding	C7LME8_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76416 (MMSYN1_0290) TruB: tRNA pseudouridine(55) synthase	AVX54702 (JCVISYN3A_0290) truB: tRNA pseudouridine(55) synthase	GO:0034337 (C-score=0.64) RNA folding	C7LME9_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76417 (MMSYN1_0291) RibF: bifunctional riboflavin kinase / FMN	AVX54703 (JCVISYN3A_0291) ribF: FAD synthetase	GO:0009398 (C-score=0.76) FMN biosynthetic process	C7LMF0_MYCML (100.00%)	3 Putative	Essential
AMW76418 (MMSYN1_0294) S15: ribosomal protein S15	AVX54704 (JCVISYN3A_0294) rpsO: 30S ribosomal protein S15	GO:0019843 (C-score=0.63) rRNA binding	C7LMF3_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76419 (MMSYN1_0296) hypothetical protein	AVX54705 (JCVISYN3A_0296) Uncharacterized protein	GO:0016286 (C-score=0.89) small conductance calcium- activated potassium channel activity	C7LMF4_MYCML (100.00%)	1 Unknown	Essential
AMW76420 (MMSYN1_0297) IF-2: translation initiation factor IF-2	AVX54706 (JCVISYN3A_0297) infB: Translation initiation factor IF-2	GO:0003924 (C-score=0.99) GTPase activity	IF2_MYCMS (99.03%)	5 Equivalog	Essential
AMW76421 (MMSYN1_0298) hypothetical protein	AVX54707 (JCVISYN3A_0298) Uncharacterized L7Ae family protein	GO:0000470 (C-score=0.60) maturation of LSU-rRNA	Q6MTP9_MYCMS (98.99%)	1 Unknown	Quasi essential
AMW76422 (MMSYN1_0299) PF04296 family protein	AVX54708 (JCVISYN3A_0299) Uncharacterized protein	GO:0016070 (C-score=0.52) RNA metabolic process	Q6MTP8_MYCMS (96.81%)	1 Unknown	Quasi essential
AMW76423 (MMSYN1_0300) NusA: Transcription termination/antitermination	AVX54709 (JCVISYN3A_0300) nusA: Transcription termination/antitermination protein NusA	GO:0031564 (C-score=0.78) transcription antitermination	Q6MTP7_MYCMS (96.75%)	5 Equivalog	Essential
AMW76424 (MMSYN1_0301) RimP: Ribosome maturation factor	AVX54710 (JCVISYN3A_0301) rimP: Ribosome assembly cofactor	GO:0000028 (C-score=1.00) ribosomal small subunit assembly	RIMP_MYCMS (96.34%)	3 Putative	Quasi essential
AMW76425 (MMSYN1_0302) hypothetical protein	AVX54711 (JCVISYN3A_0302) fre: Flavin reductase	GO:0046256 (C-score=0.54) 2,4,6-trinitrotoluene catabolic process	Q6MTP5_MYCMS (97.22%)	1 Unknown	Essential
AMW76426 (MMSYN1_0303) DNA polymerase III, alpha subunit, Gram-positive	AVX54712 (JCVISYN3A_0303) polC: PolC-type DNA polymerase III	GO:0016779 (C-score=0.70) nucleotidyltransferase activity	Q6MTP4_MYCMS (98.31%)	5 Equivalog	Essential
AMW76427 (MMSYN1_0304) CdsA: Phosphatidate cytidylyltransferase	AVX54713 (JCVISYN3A_0304) cdsA: Phosphatidate cytidylyltransferase	GO:0016024 (C-score=0.94) CDP-diacylglycerol biosynthetic process	Q6MTP3_MYCMS (97.51%)	3 Putative	Essential
AMW76428 (MMSYN1_0305) metallopeptidase family M24	AVX54714 (JCVISYN3A_0305) Uncharacterized metallopeptidase	GO:0102009 (C-score=0.75) proline dipeptidase activity	Q6MTP2_MYCMS (98.33%)	2 Generic	Quasi essential
AMW76429 (MMSYN1_0308) TrpRS: Tryptophanyl-tRNA synthetase	AVX54715 (JCVISYN3A_0308) trpRS: Tryptophan--tRNA ligase	GO:0006436 (C-score=0.92) tryptophanyl-tRNA aminoacylation	C7LMG6_MYCML (100.00%)	5 Equivalog	Essential
AMW76430 (MMSYN1_0314) EcfS: Energy coupling-factor transporter,	AVX54716 (JCVISYN3A_0314) ecfS: Uncharacterized ECF transporter S component	GO:0006935 (C-score=0.57) chemotaxis	C7LMH2_MYCML (100.00%)	2 Generic	Essential
AMW76431 (MMSYN1_0315) hypothetical protein	AVX54717 (JCVISYN3A_0315) Uncharacterized protein	GO:0090304 (C-score=0.51) nucleic acid metabolic process	Q6MTM8_MYCMS (100.00%)	1 Unknown	Quasi essential
AMW76432 (MMSYN1_0316) tkt: transketolase	AVX54718 (JCVISYN3A_0316) tkt: Transketolase	GO:0004802 (C-score=0.99) transketolase activity	C7LMH4_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76433 (MMSYN1_0317) PF03672 family protein	AVX54719 (JCVISYN3A_0317) Uncharacterized protein	GO:0019725 (C-score=0.50) cellular homeostasis	C7LMH5_MYCML (100.00%)	1 Unknown	Essential
AMW76434 (MMSYN1_0325) membrane protein, putative	AVX54720 (JCVISYN3A_0325) Uncharacterized protein	GO:0000455 (C-score=0.97) enzyme-directed rRNA pseudouridine synthesis	C7LMI2_MYCML (100.00%)	2 Generic	Essential
AMW76435 (MMSYN1_0326) hypothetical protein	AVX54721 (JCVISYN3A_0326) Uncharacterized protein	GO:0000455 (C-score=0.98) enzyme-directed rRNA pseudouridine synthesis	C7LMI3_MYCML (100.00%)	1 Unknown	Non essential
AMW76436 (MMSYN1_0327) Putative ScpA segregation and condensation	AVX54722 (JCVISYN3A_0327) scpA: Chromosome segregation protein A	GO:0051177 (C-score=0.55) meiotic sister chromatid cohesion	C7LMI4_MYCML (100.00%)	2 Generic	Essential
AMW76437 (MMSYN1_0328) Putative ScpB segregation and condensation	AVX54723 (JCVISYN3A_0328) scpB: Chromosome segregation protein B	GO:0051304 (C-score=0.56) chromosome separation	C7LMI5_MYCML (100.00%)	5 Equivalog	Essential
AMW76438 (MMSYN1_0329) Ribosomal large subunit pseudouridine synthase	AVX54724 (JCVISYN3A_0329) rluB: Uncharacterized pseudouridine synthase	GO:0000455 (C-score=1.00) enzyme-directed rRNA pseudouridine synthesis	C7LMI6_MYCML (100.00%)	4 Probable	Quasi essential
AMW76439 (MMSYN1_0330) dgk	AVX54725 (JCVISYN3A_0330) dgk: Deoxyguanosine kinase	GO:0004138 (C-score=0.58) deoxyguanosine kinase activity	C7LMI7_MYCML (100.00%)	4 Probable	Non essential
AMW76440 (MMSYN1_0332) hypothetical protein	AVX54726 (JCVISYN3A_0332) Uncharacterized protein	GO:0019136 (C-score=0.83) deoxynucleoside kinase activity	C7LMI9_MYCML (100.00%)	1 Unknown	Quasi essential
AMW76441 (MMSYN1_0338) lipoprotein, putative	AVX54727 (JCVISYN3A_0338) Uncharacterized lipoprotein	GO:0030288 (C-score=0.53) outer membrane-bounded periplasmic space	C7LMJ5_MYCML (100.00%)	1 Unknown	Essential
AMW76442 (MMSYN1_0344) Ppase	AVX54728 (JCVISYN3A_0344) ppa: Inorganic diphosphatase	GO:0004427 (C-score=0.99) inorganic diphosphatase activity	Q6MTK0_MYCMS (97.85%)	4 Probable	Essential
AMW76443 (MMSYN1_0345) EcfS: Energy coupling-factor transporter,	AVX54729 (JCVISYN3A_0345) ecfS: Uncharacterized ECF transporter S component	GO:0016020 (C-score=0.64) membrane	C7LMK1_MYCML (100.00%)	2 Generic	Essential
AMW76444 (MMSYN1_0346) hypothetical protein	AVX54730 (JCVISYN3A_0346) Uncharacterized protein	GO:0005215 (C-score=0.57) transporter activity	C7LMK2_MYCML (100.00%)	1 Unknown	Non essential
AMW76445 (MMSYN1_0347) cmk: cytidylate kinase	AVX54731 (JCVISYN3A_0347) cmk: Cytidylate kinase	GO:0046940 (C-score=0.94) nucleoside monophosphate phosphorylation	C7LMK3_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76446 (MMSYN1_0348) GTPase_EngA: ribosome- associated GTPase EngA	AVX54732 (JCVISYN3A_0348) engA: Ribosome biogenesis GTPase	GO:0043022 (C-score=0.74) ribosome binding	C7LMK4_MYCML (100.00%)	5 Equivalog	Essential
AMW76447 (MMSYN1_0350) putative DNA-binding protein HU 1	AVX54733 (JCVISYN3A_0350) hupA: DNA-binding protein	GO:0030261 (C-score=0.92) chromosome condensation	C7LMK6_MYCML (100.00%)	2 Generic	Essential
AMW76448 (MMSYN1_0352) Putative DnaD	AVX54734 (JCVISYN3A_0352) Uncharacterized protein	GO:0006269 (C-score=1.00) DNA replication, synthesis of RNA primer	C7LMK7_MYCML (100.00%)	2 Generic	Essential
AMW76449 (MMSYN1_0353) hypothetical protein	AVX54735 (JCVISYN3A_0353) Uncharacterized protein	GO:0050789 (C-score=0.50) regulation of biological process	Q6MTJ1_MYCMS (74.65%)	1 Unknown	Quasi essential
AMW76450 (MMSYN1_0359) RNase_Y: ribonuclease Y	AVX54736 (JCVISYN3A_0359) Ribonuclease Y	GO:0006139 (C-score=0.53) nucleobase-containing compound metabolic process	C7LML2_MYCML (100.00%)	5 Equivalog	Essential
AMW76451 (MMSYN1_0360) ffh: signal recognition particle protein	AVX54737 (JCVISYN3A_0360) ffh: Signal recognition particle protein	GO:0008312 (C-score=0.58) 7S RNA binding	C7LML3_MYCML (100.00%)	5 Equivalog	Essential
AMW76452 (MMSYN1_0361) RlmH: Ribosomal RNA large subunit	AVX54738 (JCVISYN3A_0361) rlmH: 23S rRNA (pseudouridine( 1915)-N3)-methyltransferase	GO:0070038 (C-score=0.85) rRNA (pseudouridine-N3-)-methy ltransferase activity	C7LML4_MYCML (100.00%)	5 Equivalog	Non essential
AMW76453 (MMSYN1_0362) S16:ribosomal protein S16	AVX54739 (JCVISYN3A_0362) rpsP: 30S ribosomal protein S16	GO:0006412 (C-score=1.00) translation	C7LML5_MYCML (100.00%)	5 Equivalog	Essential
AMW76454 (MMSYN1_0363) 16S_RimM: 16S rRNA processing protein RimM	AVX54740 (JCVISYN3A_0363) rimM: 16S rRNA processing protein	GO:0003977 (C-score=0.69) UDP-N-acetylglucosamine diphosphorylase activity	C7LML6_MYCML (100.00%)	5 Equivalog	Essential
AMW76455 (MMSYN1_0364) TrmD: tRNA (guanine(37)-N(1))- methyltransferase	AVX54741 (JCVISYN3A_0364) trmD: tRNA (guanosine(37)-N1)- methyltransferase	GO:0052906 (C-score=0.88) tRNA (guanine(37)-N(1))-methyl transferase activity	C7LML7_MYCML (100.00%)	5 Equivalog	Essential
AMW76456 (MMSYN1_0365) L19: ribosomal protein L19	AVX54742 (JCVISYN3A_0365) rplS: 50S ribosomal protein L19	GO:0006412 (C-score=0.76) translation	C7LML8_MYCML (100.00%)	5 Equivalog	Essential
AMW76457 (MMSYN1_0366) YlqF: ribosome biogenesis GTP- binding protein	AVX54743 (JCVISYN3A_0366) ylqF;rbgA: L16-binding dependent 50S subunit- maturation GTPase	GO:0042254 (C-score=0.94) ribosome biogenesis	C7LML9_MYCML (100.00%)	5 Equivalog	Essential
AMW76458 (MMSYN1_0371) ABC transporter, ATP-binding protein	AVX54744 (JCVISYN3A_0371) ywjA: Flippase A	GO:0035639 (C-score=0.62) purine ribonucleoside triphosphate binding	C7LMM4_MYCML (100.00%)	2 Generic	Essential
AMW76459 (MMSYN1_0372) ABC transporter, ATP-binding protein	AVX54745 (JCVISYN3A_0372) ywjA: Flippase B	GO:0035639 (C-score=0.72) purine ribonucleoside triphosphate binding	C7LMM5_MYCML (100.00%)	2 Generic	Essential
AMW76460 (MMSYN1_0373) hypothetical protein	AVX54746 (JCVISYN3A_0373) Uncharacterized protein	GO:0005634 (C-score=1.00) nucleus	C7LMM6_MYCML (100.00%)	1 Unknown	Essential
AMW76461 (MMSYN1_0375) hypothetical protein	AVX54747 (JCVISYN3A_0375) Uncharacterized protein	GO:0044464 (C-score=0.80) cell part	C7LMM7_MYCML (100.00%)	1 Unknown	Non essential
AMW76462 (MMSYN1_0376) hypothetical protein	AVX54748 (JCVISYN3A_0376) Uncharacterized protein	GO:0005737 (C-score=0.55) cytoplasm	C7LMM8_MYCML (100.00%)	1 Unknown	Quasi essential
AMW76463 (MMSYN1_0377) Obg_CgtA: Obg family GTPase CgtA	AVX54749 (JCVISYN3A_0377) obgE: Ribosome GTPase	GO:0005525 (C-score=0.72) GTP binding	C7LMM9_MYCML (100.00%)	5 Equivalog	Essential
AMW76464 (MMSYN1_0378) NAD+ synthetase	AVX54750 (JCVISYN3A_0378) nadE: NAD(+) synthase	GO:0008795 (C-score=0.95) NAD+ synthase activity	C7LMN0_MYCML (100.00%)	5 Equivalog	Essential
AMW76465 (MMSYN1_0379) hypothetical protein	AVX54751 (JCVISYN3A_0379) Uncharacterized protein	GO:0016021 (C-score=0.60) integral component of membrane	C7LMN1_MYCML (100.00%)	1 Unknown	Essential
AMW76466 (MMSYN1_0380) nicotinate (nicotinamide) nucleotide	AVX54752 (JCVISYN3A_0380) nadD: Nicotinate (nicotinamide) nucleotide adenylyltransferase	GO:0000309 (C-score=0.91) nicotinamide-nucleotide adenylyltransferase activity	C7LMN2_MYCML (100.00%)	5 Equivalog	Essential
AMW76467 (MMSYN1_0381) MTA/SAH-Nsdase: MTA/SAH nucleosidase	AVX54753 (JCVISYN3A_0381) mtnN: 5'-Methylthioadenosine nucleosidase	GO:0019284 (C-score=0.81) L-methionine biosynthetic process from S-adenosylmethionine	C7LMN3_MYCML (100.00%)	5 Equivalog	Non essential
AMW76468 (MMSYN1_0382) deoxynucleoside kinase	AVX54754 (JCVISYN3A_0382) dnk: Deoxynucleoside kinase	GO:0006230 (C-score=0.52) TMP biosynthetic process	C7LMN4_MYCML (100.00%)	2 Generic	Non essential
AMW76469 (MMSYN1_0387) TrmU: tRNA	AVX54755 (JCVISYN3A_0387) mnmA: tRNA 2-thiouridine(34) synthase	GO:0002143 (C-score=0.98) tRNA wobble position uridine thiolation	C7LMN8_MYCML (100.00%)	5 Equivalog	Essential
AMW76470 (MMSYN1_0388) hypothetical protein	AVX54756 (JCVISYN3A_0388) Uncharacterized protein	GO:0046483 (C-score=0.51) heterocycle metabolic process	C7LMN9_MYCML (100.00%)	1 Unknown	Essential
AMW76471 (MMSYN1_0389) PF11074 domain protein	AVX54757 (JCVISYN3A_0389) Uncharacterized protein	GO:0019752 (C-score=0.50) carboxylic acid metabolic process	C7LMP0_MYCML (100.00%)	1 Unknown	Quasi essential
AMW76472 (MMSYN1_0390) fmt: methionyl-tRNA formyltransferase	AVX54758 (JCVISYN3A_0390) fmt: Methionyl-tRNA formyltransferase	GO:0071951 (C-score=0.86) conversion of methionyl-tRNA to N-formyl-methionyl-tRNA	C7LMP1_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76473 (MMSYN1_0391) efp: translation elongation factor P	AVX54759 (JCVISYN3A_0391) efp: Elongation factor P	GO:0006414 (C-score=0.66) translational elongation	EFP_MYCMS (100.00%)	5 Equivalog	Quasi essential
AMW76474 (MMSYN1_0392) hypothetical protein	AVX54760 (JCVISYN3A_0392) Uncharacterized protein	GO:0005576 (C-score=0.80) extracellular region	Q6MTF6_MYCMS (100.00%)	1 Unknown	Quasi essential
AMW76475 (MMSYN1_0394) lon: endopeptidase La	AVX54761 (JCVISYN3A_0394) lon: Endopeptidase La	GO:0006515 (C-score=0.86) misfolded or incompletely synthesized protein catabolic process	C7LMP5_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76476 (MMSYN1_0398) lipoprotein, putative	AVX54762 (JCVISYN3A_0398) Uncharacterized lipoprotein	GO:0046539 (C-score=0.52) histamine N-methyltransferase activity	Q6MTF2_MYCMS (91.10%)	1 Unknown	Quasi essential
AMW76477 (MMSYN1_0399) efflux ABC transporter, permease protein	AVX54763 (JCVISYN3A_0399) Uncharacterized efflux ABC transporter permease	GO:0008144 (C-score=0.56) drug binding	Q6MTF1_MYCMS (97.00%)	2 Generic	Quasi essential
AMW76478 (MMSYN1_0400) DJ-1 family protein	AVX54764 (JCVISYN3A_0400) Uncharacterized protease	GO:0009228 (C-score=0.51) thiamine biosynthetic process	C7LMQ0_MYCML (100.00%)	2 Generic	Quasi essential
AMW76479 (MMSYN1_0401) peptidase, C39 family	AVX54765 (JCVISYN3A_0401) Uncharacterized peptidase	GO:0042626 (C-score=0.87) ATPase activity, coupled to transmembrane movement of substances	Q6MTE9_MYCMS (74.46%)	2 Generic	Non essential
AMW76480 (MMSYN1_0402) YbeY:rRNA maturation RNase YbeY	AVX54766 (JCVISYN3A_0402) ybeY: rRNA maturation RNase	GO:0004222 (C-score=0.64) metalloendopeptidase activity	C7LMQ1_MYCML (100.00%)	5 Equivalog	Essential
AMW76481 (MMSYN1_0403) era: GTP-binding protein Era	AVX54767 (JCVISYN3A_0403) era: Ribosome GTPase	GO:0043024 (C-score=0.60) ribosomal small subunit binding	C7LMQ2_MYCML (100.00%)	5 Equivalog	Essential
AMW76482 (MMSYN1_0404) RecO: DNA repair protein RecO	AVX54768 (JCVISYN3A_0404) recO: DNA repair protein	GO:0006302 (C-score=1.00) double-strand break repair	C7LMQ3_MYCML (100.00%)	5 Equivalog	Non essential
AMW76483 (MMSYN1_0405) GlyRS: Glycyl-tRNA synthetase	AVX54769 (JCVISYN3A_0405) glyRS: Glycine--tRNA ligase	GO:0006426 (C-score=0.74) glycyl-tRNA aminoacylation	C7LMQ4_MYCML (100.00%)	5 Equivalog	Essential
AMW76484 (MMSYN1_0406) DnaG: DNA primase	AVX54770 (JCVISYN3A_0406) dnaG: DNA primase	GO:0016779 (C-score=0.50) nucleotidyltransferase activity	C7LMQ5_MYCML (100.00%)	5 Equivalog	Essential
AMW76485 (MMSYN1_0407) RpoD: RNA polymerase sigma factor	AVX54771 (JCVISYN3A_0407) rpoD: RNA polymerase subunit sigma	GO:0016987 (C-score=0.90) sigma factor activity	C7LMQ6_MYCML (100.00%)	4 Probable	Essential
AMW76486 (MMSYN1_0408) Putative tRNA: m1A22 methyltransferase	AVX54772 (JCVISYN3A_0408) trmK;yqfN: Uncharacterized methyltransferase	GO:0016429 (C-score=0.88) tRNA (adenine-N1-)-methyltrans ferase activity	C7LMQ7_MYCML (100.00%)	2 Generic	Quasi essential
AMW76487 (MMSYN1_0409) Putative FolE	AVX54773 (JCVISYN3A_0409) Uncharacterized protein	GO:0030182 (C-score=0.79) neuron differentiation	C7LMQ8_MYCML (100.00%)	2 Generic	Quasi essential
AMW76488 (MMSYN1_0410) DEAD/DEAH box helicase	AVX54774 (JCVISYN3A_0410) Uncharacterized helicase	GO:0010501 (C-score=0.90) RNA secondary structure unwinding	C7LMQ9_MYCML (100.00%)	2 Generic	Quasi essential
AMW76489 (MMSYN1_0411) membrane protein, PF02588 family	AVX54775 (JCVISYN3A_0411) Uncharacterized protein	GO:0008144 (C-score=0.54) drug binding	C7LMR0_MYCML (100.00%)	1 Unknown	Non essential
AMW76490 (MMSYN1_0412) secDF	AVX54776 (JCVISYN3A_0412) Uncharacterized protein	GO:0015031 (C-score=0.56) protein transport	C7LMR1_MYCML (100.00%)	4 Probable	Essential
AMW76491 (MMSYN1_0413) apt: adenine phosphoribosyltransferase	AVX54777 (JCVISYN3A_0413) apt: Adenine phosphoribosyltransferase	GO:0044209 (C-score=0.87) AMP salvage	C7LMR2_MYCML (100.00%)	5 Equivalog	Essential
AMW76492 (MMSYN1_0414) RelA: GTP pyrophosphokinase	AVX54778 (JCVISYN3A_0414) relA: Guanosine-3',5'-bis(diph osphate) 3'-pyrophosphohydrolase	GO:0015969 (C-score=0.94) guanosine tetraphosphate metabolic process	C7LMR3_MYCML (100.00%)	4 Probable	Quasi essential
AMW76493 (MMSYN1_0415) SMC: chromosome segregation protein	AVX54779 (JCVISYN3A_0415) smc: Chromosome segregation protein	GO:0022402 (C-score=0.53) cell cycle process	Q6MTD2_MYCMS (98.89%)	5 Equivalog	Essential
AMW76494 (MMSYN1_0416) hypothetical protein	AVX54780 (JCVISYN3A_0416) Uncharacterized protein	GO:0044459 (C-score=0.67) plasma membrane part	C7LMQ4_MYCML (12.30%)	1 Unknown	Quasi essential
AMW76495 (MMSYN1_0418) RNaseIII: ribonuclease III	AVX54781 (JCVISYN3A_0418) Ribonuclease III	GO:0031054 (C-score=0.61) pre-miRNA processing	RNC_MYCMS (99.14%)	5 Equivalog	Essential
AMW76496 (MMSYN1_0419) PlsX: fatty acid/phospholipid synthesis protein	AVX54782 (JCVISYN3A_0419) plsX: Phosphate acyltransferase	GO:0006633 (C-score=0.67) fatty acid biosynthetic process	PLSX_MYCMS (99.10%)	5 Equivalog	Essential
AMW76497 (MMSYN1_0420) yloV:DAK2 domain fusion protein YloV	AVX54783 (JCVISYN3A_0420) fakA: Fatty acid kinase subunit A	GO:0019563 (C-score=0.59) glycerol catabolic process	Q6MTC7_MYCMS (98.35%)	2 Generic	Essential
AMW76498 (MMSYN1_0421) alkaline shock protein Asp23 family protein	AVX54784 (JCVISYN3A_0421) Uncharacterized protein	GO:0044249 (C-score=0.55) cellular biosynthetic process	Q6MTC6_MYCMS (97.61%)	1 Unknown	Quasi essential
AMW76499 (MMSYN1_0422) L28: ribosomal protein L28	AVX54785 (JCVISYN3A_0422) rpmB: 50S ribosomal protein L28	GO:0006412 (C-score=1.00) translation	C7LMS1_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76500 (MMSYN1_0424) hypothetical protein	AVX54786 (JCVISYN3A_0424) Uncharacterized protein	GO:0003777 (C-score=0.81) microtubule motor activity	C7LMS2_MYCML (100.00%)	1 Unknown	Essential
AMW76501 (MMSYN1_0425) PstS: phosphate binding protein	AVX54787 (JCVISYN3A_0425) pstS: Phosphate ABC transporter substrate-binding protein	GO:0016036 (C-score=0.88) cellular response to phosphate starvation	C7LMS3_MYCML (100.00%)	4 Probable	Essential
AMW76502 (MMSYN1_0426) PstA, PstC: phosphate ABC transporter, permease	AVX54788 (JCVISYN3A_0426) pstA: Phosphate ABC transporter permease	GO:0043225 (C-score=0.52) anion transmembrane- transporting ATPase activity	C7LMS4_MYCML (100.00%)	5 Equivalog	Essential
AMW76503 (MMSYN1_0427) PstB: phosphate ABC transporter, ATP-binding	AVX54789 (JCVISYN3A_0427) pstB: Phosphate ABC transporter ATP-binding protein	GO:0030554 (C-score=0.80) adenyl nucleotide binding	C7MJI3_MYCML (100.00%)	5 Equivalog	Essential
AMW76504 (MMSYN1_0428) phoU: phosphate transport system regulatory	AVX54790 (JCVISYN3A_0428) phoU: Phosphate transport system regulatory protein	GO:2000186 (C-score=0.86) negative regulation of phosphate transmembrane transport	C7MJI4_MYCML (100.00%)	5 Equivalog	Essential
AMW76505 (MMSYN1_0429) FtsY: signal recognition particle-docking	AVX54791 (JCVISYN3A_0429) ftsY: Signal recognition particle-docking protein	GO:0006614 (C-score=0.92) SRP-dependent cotranslational protein targeting to membrane	C7LMS7_MYCML (100.00%)	5 Equivalog	Essential
AMW76506 (MMSYN1_0430) Putative Sigma3 and sigma4 domains of RNA	AVX54792 (JCVISYN3A_0430) Uncharacterized DNA-binding protein	GO:0006355 (C-score=0.90) regulation of transcription, DNA-templated	C7LMS8_MYCML (100.00%)	2 Generic	Essential
AMW76507 (MMSYN1_0431) putative metallophosphoesterase	AVX54793 (JCVISYN3A_0431) Uncharacterized metallophosphoesterase	GO:0008663 (C-score=0.64) 2',3'-cyclic-nucleotide 2'-phosphodiesterase activity	C7LMS9_MYCML (100.00%)	2 Generic	Essential
AMW76508 (MMSYN1_0432) MetK: methionine adenosyltransferase	AVX54794 (JCVISYN3A_0432) metK: Methionine adenosyltransferase	GO:0006556 (C-score=0.94) S-adenosylmethionine biosynthetic process	C7LMT0_MYCML (100.00%)	5 Equivalog	Essential
AMW76509 (MMSYN1_0433) CutC family protein	AVX54795 (JCVISYN3A_0433) cutC: Uncharacterized protein	GO:0006878 (C-score=0.82) cellular copper ion homeostasis	C7LMT1_MYCML (100.00%)	1 Unknown	Non essential
AMW76510 (MMSYN1_0434) gid_trmFO: tRNA:m(5)U-54 methyltransferase	AVX54796 (JCVISYN3A_0434) rlmFO: 23S rRNA (uridine(1939) -m5)-methyltransferase	GO:0002098 (C-score=0.95) tRNA wobble uridine modification	C7LMT2_MYCML (100.00%)	5 Equivalog	Non essential
AMW76511 (MMSYN1_0435) ManA: mannose-6-phosphate isomerase, class I	AVX54797 (JCVISYN3A_0435) manA: Mannose-6-phosphate isomerase	GO:0004476 (C-score=1.00) mannose-6-phosphate isomerase activity	C7LMT3_MYCML (100.00%)	5 Equivalog	Non essential
AMW76512 (MMSYN1_0437) YhaM:putative 3'-5' exoribonuclease	AVX54798 (JCVISYN3A_0437) yhaM: Putative 3'-5' exoribonuclease	GO:0042578 (C-score=0.54) phosphoric ester hydrolase activity	C7LMT5_MYCML (100.00%)	2 Generic	Essential
AMW76513 (MMSYN1_0438) Histidine triad (HIT) hydrolase-like protein	AVX54799 (JCVISYN3A_0438) hinT: Uncharacterized protein	GO:0009166 (C-score=0.54) nucleotide catabolic process	Q6MTB0_MYCMS (96.97%)	2 Generic	Non essential
AMW76514 (MMSYN1_0439) lipoprotein, putative	AVX54800 (JCVISYN3A_0439) Uncharacterized lipoprotein	GO:0005739 (C-score=0.50) mitochondrion	Q6MTA9_MYCMS (90.03%)	1 Unknown	Essential
AMW76515 (MMSYN1_0440) lipoprotein, putative	AVX54801 (JCVISYN3A_0440) Uncharacterized lipoprotein	GO:0005540 (C-score=0.91) hyaluronic acid binding	Q6MTA8_MYCMS (88.29%)	1 Unknown	Essential
AMW76516 (MMSYN1_0441) IscS: Cysteine desulfurase	AVX54802 (JCVISYN3A_0441) iscS: Cysteine desulfurase	GO:0009000 (C-score=0.80) selenocysteine lyase activity	C7LMT9_MYCML (100.00%)	3 Putative	Essential
AMW76517 (MMSYN1_0442) iscU: Iron-sulfur cluster assembly scaffold	AVX54803 (JCVISYN3A_0442) iscU: Iron-sulfur cluster assembly scaffold protein	GO:0044571 (C-score=0.99) [2Fe-2S] cluster assembly	C7LMU0_MYCML (100.00%)	3 Putative	Essential
AMW76518 (MMSYN1_0443) YqgN: 5-formyltetrahydrofolate cyclo-ligase	AVX54804 (JCVISYN3A_0443) yggN: 5-Formyltetrahydrofolate cyclo-ligase	GO:0030272 (C-score=1.00) 5-formyltetrahydrofolate cyclo-ligase activity	C7LMU1_MYCML (100.00%)	5 Equivalog	Essential
AMW76519 (MMSYN1_0444) peptidase family M13	AVX54805 (JCVISYN3A_0444) Uncharacterized peptidase	GO:0016486 (C-score=0.52) peptide hormone processing	C7LMU2_MYCML (100.00%)	2 Generic	Quasi essential
AMW76520 (MMSYN1_0445) gpi: Glucose-6-phosphate isomerase	AVX54806 (JCVISYN3A_0445) pgi: Glucose-6-phosphate isomerase	GO:0004347 (C-score=1.00) glucose-6-phosphate isomerase activity	C7LMU3_MYCML (100.00%)	4 Probable	Essential
AMW76521 (MMSYN1_0447) Putative dUTP diphosphatase	AVX54807 (JCVISYN3A_0447) dut: dUTP diphosphatase	GO:0004170 (C-score=0.70) dUTP diphosphatase activity	C7LMU5_MYCML (100.00%)	2 Generic	Essential
AMW76522 (MMSYN1_0448) trmH-like	AVX54808 (JCVISYN3A_0448) Uncharacterized rRNA methyltransferase	GO:0070039 (C-score=0.53) rRNA (guanosine-2'-O-)-methylt ransferase activity	C7LMU6_MYCML (100.00%)	3 Putative	Essential
AMW76523 (MMSYN1_0451) putative glyceraldehyde-3-phosphate	AVX54809 (JCVISYN3A_0451) gapdh: Glyceraldehyde-3-phosphate dehydrogenase	GO:0004029 (C-score=0.94) aldehyde dehydrogenase (NAD) activity	C7LMU9_MYCML (100.00%)	2 Generic	Essential
AMW76524 (MMSYN1_0452) DNA topoisomerase IV, B subunit	AVX54810 (JCVISYN3A_0452) parE: DNA topoisomerase IV subunit B	GO:0003918 (C-score=1.00) DNA topoisomerase type II (ATP-hydrolyzing) activity	C7LMV0_MYCML (100.00%)	5 Equivalog	Essential
AMW76525 (MMSYN1_0453) DNA topoisomerase IV, A subunit	AVX54811 (JCVISYN3A_0453) parC: DNA topoisomerase IV subunit A	GO:0003918 (C-score=0.99) DNA topoisomerase type II (ATP-hydrolyzing) activity	C7LMV1_MYCML (100.00%)	5 Equivalog	Essential
AMW76526 (MMSYN1_0475) L-LDH-NAD: L-lactate dehydrogenase	AVX54812 (JCVISYN3A_0475) ldh: L-lactate dehydrogenase	GO:0004459 (C-score=0.93) L-lactate dehydrogenase activity	LDH_MYCMS (98.74%)	5 Equivalog	Essential
AMW76527 (MMSYN1_0478) hypothetical protein	AVX54813 (JCVISYN3A_0478) Uncharacterized protein	GO:0005886 (C-score=0.54) plasma membrane	Q6MT75_MYCMS (95.36%)	1 Unknown	Essential
AMW76528 (MMSYN1_0479) peptidase	AVX54814 (JCVISYN3A_0479) Uncharacterized peptidase	GO:0032386 (C-score=0.57) regulation of intracellular transport	Q6MT73_MYCMS (66.51%)	2 Generic	Quasi essential
AMW76529 (MMSYN1_0481) lipoprotein, putative	AVX54815 (JCVISYN3A_0481) Uncharacterized lipoprotein	GO:0050794 (C-score=0.64) regulation of cellular process	Q6MT70_MYCMS (73.46%)	1 Unknown	Non essential
AMW76530 (MMSYN1_0482) S21p: ribosomal protein S21	AVX54816 (JCVISYN3A_0482) rpsU: 30S ribosomal protein S21	GO:0007154 (C-score=0.69) cell communication	C7LMX8_MYCML (100.00%)	5 Equivalog	Essential
AMW76531 (MMSYN1_0154) cytosol aminopeptidase family, catalytic domain	AVX54817 (JCVISYN3A_0154) Uncharacterized peptidase	GO:0004177 (C-score=0.95) aminopeptidase activity	C7LM24_MYCML (100.00%)	3 Putative	Quasi essential
AMW76532 (MMSYN1_0493) putative dipeptidase	AVX54818 (JCVISYN3A_0493) Uncharacterized peptidase	GO:0070573 (C-score=0.95) metallodipeptidase activity	Q6MT56_MYCMS (98.02%)	2 Generic	Quasi essential
AMW76533 (MMSYN1_0494) putative N-acetylmannosamine-6-P epimerase	AVX54819 (JCVISYN3A_0494) nanE: N-acetylmannosamine-6-ph osphate 2-epimerase	GO:0047465 (C-score=0.83) N-acylglucosamine-6-phosphate 2-epimerase activity	NANE_MYCMS (99.12%)	3 Putative	Non essential
AMW76534 (MMSYN1_0495) ROK family protein	AVX54820 (JCVISYN3A_0495) Uncharacterized kinase	GO:0051156 (C-score=0.86) glucose 6-phosphate metabolic process	Q6MT54_MYCMS (97.59%)	2 Generic	Non essential
AMW76535 (MMSYN1_0499) L27: ribosomal protein L27	AVX54821 (JCVISYN3A_0499) rpmA: 50S ribosomal protein L27	GO:0006412 (C-score=0.84) translation	RL27_MYCMS (100.00%)	5 Equivalog	Essential
AMW76536 (MMSYN1_0500) PF04327 family protein	AVX54822 (JCVISYN3A_0500) prp;ysxB: Maturation protease for ribosomal protein L27	GO:0034641 (C-score=0.52) cellular nitrogen compound metabolic process	Q6MT49_MYCMS (91.10%)	1 Unknown	Essential
AMW76537 (MMSYN1_0501) L21: ribosomal protein L21	AVX54823 (JCVISYN3A_0501) rplU: 50S ribosomal protein L21	GO:0019843 (C-score=0.76) rRNA binding	RL21_MYCMS (92.00%)	5 Equivalog	Essential
AMW76538 (MMSYN1_0503) hypothetical protein	AVX54824 (JCVISYN3A_0503) Uncharacterized protein	GO:0009279 (C-score=0.50) cell outer membrane	Q6MT47_MYCMS (83.15%)	1 Unknown	Quasi essential
AMW76539 (MMSYN1_0504) Putative 16S rRNA	AVX54825 (JCVISYN3A_0504) rsmI: 16S rRNA (cytidine(1402) -2'-O)-methyltransferase	GO:0070677 (C-score=0.51) rRNA (cytosine-2'-O-)-methyltr ansferase activity	Q6MT46_MYCMS (93.22%)	2 Generic	Non essential
AMW76540 (MMSYN1_0505) lipoprotein, putative	AVX54827 (JCVISYN3A_0505) Uncharacterized lipoprotein	GO:0032559 (C-score=0.51) adenyl ribonucleotide binding	C7LKL7_MYCML (100.00%)	2 Generic	Non essential
AMW76541 (MMSYN1_0511) hypothetical protein	AVX54828 (JCVISYN3A_0511) Uncharacterized protein	GO:0046474 (C-score=0.51) glycerophospholipid biosynthetic process	Q6MT37_MYCMS (94.20%)	1 Unknown	Essential
AMW76542 (MMSYN1_0512) PlsC: 1-acyl-sn- glycerol-3-phosphate	AVX54829 (JCVISYN3A_0512) plsC: Acyl-phosphate glycerol 3-phosphate acyltransferase	GO:0003841 (C-score=0.58) 1-acylglycerol-3-phosphate O-acyltransferase activity	Q6MT36_MYCMS (98.88%)	3 Putative	Essential
AMW76543 (MMSYN1_0513) AcpS: 4'-phosphopantetheinyl transferase	AVX54830 (JCVISYN3A_0513) acpS: ACP synthase	GO:0008897 (C-score=0.91) holo-[acyl-carrier-protein] synthase activity	ACPS_MYCMS (100.00%)	4 Probable	Essential
AMW76544 (MMSYN1_0515) dctD: Cytidine and deoxycytidylate deaminase	AVX54831 (JCVISYN3A_0515) dctD: Cytidine deaminase	GO:0004132 (C-score=0.60) dCMP deaminase activity	Q6MT32_MYCMS (100.00%)	4 Probable	Quasi essential
AMW76545 (MMSYN1_0516) membrane protein, putative	AVX54832 (JCVISYN3A_0516) Uncharacterized protein	GO:0019239 (C-score=0.65) deaminase activity	Q6MT31_MYCMS (98.04%)	1 Unknown	Quasi essential
AMW76546 (MMSYN1_0517) RluD: Ribosomal large subunit pseudouridine	AVX54833 (JCVISYN3A_0517) rluD: Uncharacterized RNA pseudouridine synthase	GO:0000455 (C-score=0.74) enzyme-directed rRNA pseudouridine synthesis	Q6MT30_MYCMS (98.06%)	3 Putative	Non essential
AMW76547 (MMSYN1_0518) lspA: Lipoprotein signal peptidase	AVX54834 (JCVISYN3A_0518) lspA: Lipoprotein signal peptidase	GO:0006465 (C-score=0.60) signal peptide processing	Q6MT29_MYCMS (96.04%)	3 Putative	Essential
AMW76548 (MMSYN1_0519) IleRS: Isoleucyl-tRNA synthetase	AVX54835 (JCVISYN3A_0519) ileRS: Isoleucine--tRNA ligase	GO:0006428 (C-score=0.68) isoleucyl-tRNA aminoacylation	SYI_MYCMS (97.81%)	5 Equivalog	Essential
AMW76549 (MMSYN1_0523) ftsA: Cell division protein	AVX54839 (JCVISYN3A_0523) ftsA: Cell division protein	GO:0006950 (C-score=0.57) response to stress	Q6MT24_MYCMS (83.99%)	3 Putative	Non essential
AMW76550 (MMSYN1_0524) RsmH: 16S rRNA m4C1402	AVX54840 (JCVISYN3A_0524) rsmH;mraW: 16S rRNA (cytosine( 1402)-N(4))-methyltransferase	GO:0071424 (C-score=1.00) rRNA (cytosine-N4-)-methyltran sferase activity	RSMH_MYCMS (99.35%)	5 Equivalog	Non essential
AMW76551 (MMSYN1_0525) MraZ: Transcriptional regulator	AVX54841 (JCVISYN3A_0525) mraZ: Cell division/cell wall cluster transcriptional repressor	GO:0040007 (C-score=0.57) growth	MRAZ_MYCMS (96.99%)	5 Equivalog	Non essential
AMW76552 (MMSYN1_0526) L32: ribosomal protein L32	AVX54842 (JCVISYN3A_0526) rpmF: 50S ribosomal protein L32	GO:0006412 (C-score=0.90) translation	RL32_MYCMS (100.00%)	5 Equivalog	Essential
AMW76553 (MMSYN1_0528) PheRS: Phenylalanyl-tRNA synthetase subunit	AVX54844 (JCVISYN3A_0528) pheRS: Phenylalanine--tRNA ligase subunit beta	GO:0006432 (C-score=1.00) phenylalanyl-tRNA aminoacylation	SYFB_MYCMS (98.49%)	5 Equivalog	Essential
AMW76554 (MMSYN1_0529) PheRS: Phenylalanyl-tRNA synthetase subunit	AVX54845 (JCVISYN3A_0529) pheS: Phenylalanine--tRNA ligase subunit alpha	GO:0006432 (C-score=1.00) phenylalanyl-tRNA aminoacylation	SYFA_MYCMS (96.86%)	5 Equivalog	Essential
AMW76555 (MMSYN1_0530) hypothetical protein	AVX54846 (JCVISYN3A_0530) Uncharacterized protein	GO:0016070 (C-score=0.50) RNA metabolic process	Q6MT16_MYCMS (96.53%)	1 Unknown	Quasi essential
AMW76556 (MMSYN1_0531) efflux ABC transporter, permease protein	(NA)	GO:0016070 (C-score=0.65) RNA metabolic process	Q6MT15_MYCMS (98.25%)	1 Unknown
AMW76557 (MMSYN1_0535) ArgRS: Arginyl-tRNA synthetase	AVX54847 (JCVISYN3A_0535) argRS: Arginine--tRNA ligase	GO:0006420 (C-score=0.99) arginyl-tRNA aminoacylation	SYR_MYCMS (97.83%)	5 Equivalog	Essential
AMW76558 (MMSYN1_0536) frr: ribosome recycling factor	AVX54848 (JCVISYN3A_0536) frr: Ribosome recycling factor	GO:0002184 (C-score=0.85) cytoplasmic translational termination	RRF_MYCMS (99.45%)	5 Equivalog	Essential
AMW76559 (MMSYN1_0537) PyrH_bact: UMP kinase	AVX54849 (JCVISYN3A_0537) pyrH: UMP kinase	GO:0044210 (C-score=0.92) 'de novo' CTP biosynthetic process	Q6MT13_MYCMS (75.80%)	5 Equivalog	Essential
AMW76560 (MMSYN1_0539) tsf: translation elongation factor Ts	AVX54851 (JCVISYN3A_0539) tsf: Translation elongation factor Ts	GO:0006414 (C-score=0.99) translational elongation	EFTS_MYCMS (98.64%)	5 Equivalog	Essential
AMW76561 (MMSYN1_0540) S2: ribosomal protein S2	AVX54852 (JCVISYN3A_0540) rpsB: 30S ribosomal protein S2	GO:0006412 (C-score=1.00) translation	Q6MT08_MYCMS (58.57%)	5 Equivalog	Essential
AMW76562 (MMSYN1_0541) DnaJ: chaperone protein DnaJ	AVX54853 (JCVISYN3A_0541) dnaJ: Molecular chaperone	GO:0034641 (C-score=0.53) cellular nitrogen compound metabolic process	DNAJ_MYCMS (99.19%)	5 Equivalog	Essential
AMW76563 (MMSYN1_0542) DnaK: chaperone protein DnaK	AVX54854 (JCVISYN3A_0542) dnaK: Molecular chaperone	GO:0051082 (C-score=0.59) unfolded protein binding	DNAK_MYCMS (99.66%)	5 Equivalog	Essential
AMW76564 (MMSYN1_0543) grpE: Nucleotide exchange factor for DnaK	AVX54855 (JCVISYN3A_0543) grpE: Nucleotide exchange factor	GO:0000774 (C-score=0.66) adenyl-nucleotide exchange factor activity	GRPE_MYCMS (93.50%)	4 Probable	Essential
AMW76565 (MMSYN1_0544) HrcA: heat-inducible transcription repressor	AVX54856 (JCVISYN3A_0544) hrcA: Heat-inducible transcription repressor	GO:0045892 (C-score=1.00) negative regulation of transcription, DNA-templated	HRCA_MYCMS (99.71%)	5 Equivalog	Essential
AMW76566 (MMSYN1_0545) ClpB: chaperone protein ClpB	AVX54857 (JCVISYN3A_0545) clpB: ATP-dependent Clp protease subunit B	GO:0009628 (C-score=0.55) response to abiotic stimulus	Q6MT03_MYCMS (99.72%)	4 Probable	Essential
AMW76567 (MMSYN1_0599) hypothetical protein	AVX54863 (JCVISYN3A_0599) Uncharacterized protein	GO:0006810 (C-score=0.51) transport	Q6MU48_MYCMS (97.83%)	1 Unknown	Non essential
AMW76568 (MMSYN1_0600) RnjA: Ribonuclease J1	AVX54864 (JCVISYN3A_0600) rnjA: Ribonuclease J	GO:0004534 (C-score=0.86) 5'-3' exoribonuclease activity	C7LKU7_MYCML (100.00%)	4 Probable	Essential
AMW76569 (MMSYN1_0601) membrane protein, putative	AVX54865 (JCVISYN3A_0601) Uncharacterized protein	GO:0051307 (C-score=0.92) meiotic chromosome separation	C7LKU8_MYCML (100.00%)	2 Generic	Non essential
AMW76570 (MMSYN1_0606) pgk: phosphoglycerate kinase	AVX54868 (JCVISYN3A_0606) pgk: Phosphoglycerate kinase	GO:0004618 (C-score=0.99) phosphoglycerate kinase activity	PGK_MYCMS (98.76%)	4 Probable	Essential
AMW76571 (MMSYN1_0607) GAPDH-I: glyceraldehyde-3-phosphate	AVX54869 (JCVISYN3A_0607) gapDH: Type I glyceraldehyde-3-phosphate dehydrogenase	GO:0004365 (C-score=1.00) glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity	Q6MSU0_MYCMS (98.82%)	5 Equivalog	Essential
AMW76572 (MMSYN1_0608) DnaI: Primosomal protein	AVX54870 (JCVISYN3A_0608) dnaI: Primosomal protein	GO:0006261 (C-score=0.53) DNA-dependent DNA replication	Q6MST9_MYCMS (98.72%)	4 Probable	Essential
AMW76573 (MMSYN1_0609) DnaB: Replicative DNA helicase	AVX54871 (JCVISYN3A_0609) dnaB: Chromosome replication initiation protein	GO:0006270 (C-score=1.00) DNA replication initiation	Q6MST8_MYCMS (98.73%)	3 Putative	Essential
AMW76574 (MMSYN1_0611) PolA: DNA polymerase I	AVX54873 (JCVISYN3A_0611) polA: DNA polymerase I	GO:0008409 (C-score=0.92) 5'-3' exonuclease activity	Q6MST6_MYCMS (97.15%)	5 Equivalog	Essential
AMW76575 (MMSYN1_0612) DnaE: DNA polymerase III subunit alpha	AVX54874 (JCVISYN3A_0612) dnaE: DNA polymerase III subunit alpha	GO:0003887 (C-score=0.98) DNA-directed DNA polymerase activity	Q6MST5_MYCMS (95.95%)	4 Probable	Essential
AMW76576 (MMSYN1_0613) TyrRS: Tyrosyl-tRNA synthetase	AVX54875 (JCVISYN3A_0613) tyrRS: Tyrosine--tRNA ligase	GO:0006437 (C-score=0.87) tyrosyl-tRNA aminoacylation	SYY_MYCMS (98.31%)	5 Equivalog	Essential
AMW76577 (MMSYN1_0614) PncB: Nicotinate phosphoribosyltransferase	AVX54876 (JCVISYN3A_0614) pncB: Nicotinate phosphoribosyltransferase	GO:0004516 (C-score=0.99) nicotinate phosphoribosyltransferase activity	Q6MST2_MYCMS (95.66%)	4 Probable	Essential
AMW76578 (MMSYN1_0615) tRNA binding domain protein	AVX54877 (JCVISYN3A_0615) Uncharacterized protein	GO:0004826 (C-score=0.75) phenylalanine-tRNA ligase activity	Q6MST1_MYCMS (94.17%)	2 Generic	Quasi essential
AMW76579 (MMSYN1_0616) FakB: Fatty acid kinase subunit	AVX54878 (JCVISYN3A_0616) fakB: Fatty acid binding protein	GO:0008289 (C-score=0.96) lipid binding	C7LKW1_MYCML (100.00%)	3 Putative	Essential
AMW76580 (MMSYN1_0617) FakB: Fatty acid kinase subunit	AVX54879 (JCVISYN3A_0617) fakB: Fatty acid binding protein	GO:0008289 (C-score=0.94) lipid binding	C7LKW2_MYCML (100.00%)	3 Putative	Quasi essential
AMW76581 (MMSYN1_0620) transcription factor, Fur family	AVX54880 (JCVISYN3A_0620) Uncharacterized transcriptional regulator	GO:0000035 (C-score=0.65) acyl binding	Q6MSS8_MYCMS (100.00%)	2 Generic	Non essential
AMW76582 (MMSYN1_0621) AcpA: Acyl carrier protein	AVX54881 (JCVISYN3A_0621) acpA: Acyl carrier protein	GO:0001217 (C-score=0.66) bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding	Q6MSS7_MYCMS (100.00%)	4 Probable	Essential
AMW76583 (MMSYN1_0632) hypothetical protein	(NA)	GO:0043169 (C-score=0.57) cation binding	Q6MSR2_MYCMS (56.78%)	1 Unknown
AMW76584 (MMSYN1_0634) LeuRS: Leucyl-tRNA synthetase	AVX54884 (JCVISYN3A_0634) leuRS: Leucine--tRNA ligase	GO:0006429 (C-score=0.64) leucyl-tRNA aminoacylation	SYL_MYCMS (96.27%)	5 Equivalog	Essential
AMW76585 (MMSYN1_0636) lipoprotein, putative	AVX54885 (JCVISYN3A_0636) Uncharacterized lipoprotein	GO:0005048 (C-score=0.51) signal sequence binding	Q6MSQ9_MYCMS (72.37%)	1 Unknown	Essential
AMW76586 (MMSYN1_0637) S5: ribosomal protein S5	AVX54886 (JCVISYN3A_0637) rpsI: 30S ribosomal protein S9	GO:0000462 (C-score=0.99) maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU- rRNA)	Q6MSQ7_MYCMS (99.24%)	4 Probable	Essential
AMW76587 (MMSYN1_0638) L13: ribosomal protein L13	AVX54887 (JCVISYN3A_0638) rplM: 50S ribosomal protein L13	GO:0017148 (C-score=0.74) negative regulation of translation	RL13_MYCMS (98.68%)	5 Equivalog	Essential
AMW76588 (MMSYN1_0639) efflux ABC transporter, permease protein	AVX54888 (JCVISYN3A_0639) Uncharacterized efflux ABC transporter permease	GO:0016757 (C-score=0.50) transferase activity, transferring glycosyl groups	Q6MSQ5_MYCMS (98.08%)	2 Generic	Essential
AMW76589 (MMSYN1_0640) putative tRNA pseudouridine(38-40) synthase	AVX54889 (JCVISYN3A_0640) truA: tRNA pseudouridine(38-40) synthase	GO:0031119 (C-score=0.85) tRNA pseudouridine synthesis	TRUA_MYCMS (98.01%)	2 Generic	Quasi essential
AMW76590 (MMSYN1_0641) EcfT: Energy-coupling factor transporter, T	AVX54890 (JCVISYN3A_0641) ecfT: ECF transporter T component	GO:0032218 (C-score=0.73) riboflavin transport	Q6MSQ3_MYCMS (97.62%)	4 Probable	Essential
AMW76591 (MMSYN1_0642) EcfA: Energy-coupling factor transporter, A	AVX54891 (JCVISYN3A_0642) ecfA: ECF transporter ATPase	GO:0042626 (C-score=0.92) ATPase activity, coupled to transmembrane movement of substances	ECFA2_MYCMS (99.01%)	4 Probable	Essential
AMW76592 (MMSYN1_0643) EcfA: Energy-coupling factor transporter, A	AVX54892 (JCVISYN3A_0643) ecfA: ECF transporter ATPase	GO:0042626 (C-score=0.94) ATPase activity, coupled to transmembrane movement of substances	ECFA1_MYCMS (98.77%)	4 Probable	Essential
AMW76593 (MMSYN1_0644) L17: ribosomal protein L17	AVX54893 (JCVISYN3A_0644) rplQ: 50S ribosomal protein L17	GO:0006412 (C-score=0.75) translation	RL17_MYCMS (100.00%)	5 Equivalog	Essential
AMW76594 (MMSYN1_0645) RpoA: DNA-directed RNA polymerase, alpha	AVX54894 (JCVISYN3A_0645) rpoA: DNA-directed RNA polymerase subunit alpha	GO:0003899 (C-score=0.90) DNA-directed RNA polymerase activity	RPOA_MYCMS (99.68%)	5 Equivalog	Essential
AMW76595 (MMSYN1_0646) S11: 30S ribosomal protein S11	AVX54895 (JCVISYN3A_0646) rpsK: 30S ribosomal protein S11	GO:0048027 (C-score=0.99) mRNA 5'-UTR binding	RS11_MYCMS (100.00%)	5 Equivalog	Essential
AMW76596 (MMSYN1_0647) S13: 30S ribosomal protein S13	AVX54896 (JCVISYN3A_0647) rpsM: 30S ribosomal protein S13	GO:0000049 (C-score=0.53) tRNA binding	RS13_MYCMS (99.17%)	5 Equivalog	Essential
AMW76597 (MMSYN1_0648) L36: ribosomal protein L36	AVX54897 (JCVISYN3A_0648) rpmJ: 50S ribosomal protein L36	GO:0042254 (C-score=0.97) ribosome biogenesis	C7LMU6_MYCML (7.53%)	5 Equivalog	Essential
AMW76598 (MMSYN1_0649) InfA: translation initiation factor IF-1	AVX54898 (JCVISYN3A_0649) infA: Translation initiation factor IF-1	GO:0043022 (C-score=0.89) ribosome binding	Q6MTX9_MYCMS (11.90%)	5 Equivalog	Essential
AMW76599 (MMSYN1_0650) met_pdase_I: methionine aminopeptidase, type I	AVX54899 (JCVISYN3A_0650) map: Type I methionyl aminopeptidase	GO:0070084 (C-score=0.92) protein initiator methionine removal	Q6MSP6_MYCMS (99.20%)	5 Equivalog	Essential
AMW76600 (MMSYN1_0651) adk: Adenylate kinase	AVX54900 (JCVISYN3A_0651) adk: Adenylate kinase	GO:0044209 (C-score=0.95) AMP salvage	KAD_MYCMS (98.59%)	4 Probable	Essential
AMW76601 (MMSYN1_0652) SecY: Preprotein translocase, SecY subunit	AVX54901 (JCVISYN3A_0652) secY: Preprotein translocase subunit	GO:0043952 (C-score=0.69) protein transport by the Sec complex	Q6MSP4_MYCMS (99.38%)	5 Equivalog	Essential
AMW76602 (MMSYN1_0653) L15: ribosomal protein L15	AVX54902 (JCVISYN3A_0653) rplO: 50S ribosomal protein L15	GO:0019843 (C-score=0.57) rRNA binding	RL15_MYCMS (100.00%)	5 Equivalog	Quasi essential
AMW76603 (MMSYN1_0654) S5: ribosomal protein S5	AVX54903 (JCVISYN3A_0654) rpsE: 30S ribosomal protein S5	GO:0006412 (C-score=0.98) translation	RS5_MYCMS (98.43%)	5 Equivalog	Essential
AMW76604 (MMSYN1_0655) L18: ribosomal protein L18	AVX54904 (JCVISYN3A_0655) rplR: 50S ribosomal protein L18	GO:0008097 (C-score=1.00) 5S rRNA binding	RL18_MYCMS (100.00%)	5 Equivalog	Essential
AMW76605 (MMSYN1_0656) L6: ribosomal protein L6	AVX54905 (JCVISYN3A_0656) rplF: 50S ribosomal protein L6	GO:0002181 (C-score=0.99) cytoplasmic translation	RL6_MYCMS (99.44%)	5 Equivalog	Essential
AMW76606 (MMSYN1_0657) S8: ribosomal protein S8	AVX54906 (JCVISYN3A_0657) rpsH: 30S ribosomal protein S8	GO:0043488 (C-score=0.53) regulation of mRNA stability	RS8_MYCMS (100.00%)	4 Probable	Essential
AMW76607 (MMSYN1_0658) S14: ribosomal protein S14	AVX54907 (JCVISYN3A_0658) rpsN: 30S ribosomal protein S14	GO:0006412 (C-score=0.99) translation	C7LM18_MYCML (15.00%)	4 Probable	Essential
AMW76608 (MMSYN1_0659) L5: ribosomal protein L5	AVX54908 (JCVISYN3A_0659) rplE: 50S ribosomal protein L5	GO:0000027 (C-score=0.98) ribosomal large subunit assembly	RL5_MYCMS (98.89%)	4 Probable	Essential
AMW76609 (MMSYN1_0660) L24: ribosomal protein L24	AVX54909 (JCVISYN3A_0660) rplX: 50S ribosomal protein L24	GO:0006412 (C-score=0.99) translation	RL24_MYCMS (99.07%)	5 Equivalog	Essential
AMW76610 (MMSYN1_0661) L14: ribosomal protein L14	AVX54910 (JCVISYN3A_0661) rplN: 50S ribosomal protein L14	GO:0070180 (C-score=0.99) large ribosomal subunit rRNA binding	RL14_MYCMS (100.00%)	5 Equivalog	Essential
AMW76611 (MMSYN1_0662) S17: 30S ribosomal protein S17	AVX54911 (JCVISYN3A_0662) rpsQ: 30S ribosomal protein S17	GO:0006412 (C-score=1.00) translation	RS17_MYCMS (100.00%)	5 Equivalog	Essential
AMW76612 (MMSYN1_0663) L29: ribosomal protein L29	AVX54912 (JCVISYN3A_0663) rpmC: 50S ribosomal protein L29	GO:0000463 (C-score=0.66) maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU- rRNA)	Q6MSN3_MYCMS (100.00%)	5 Equivalog	Essential
AMW76613 (MMSYN1_0664) L16: ribosomal protein L16	AVX54913 (JCVISYN3A_0664) rplP: 50S ribosomal protein L16	GO:0019843 (C-score=1.00) rRNA binding	RL16_MYCMS (99.27%)	5 Equivalog	Essential
AMW76614 (MMSYN1_0665) S3: ribosomal protein S3	AVX54914 (JCVISYN3A_0665) rpsC: 30S ribosomal protein S3	GO:0006281 (C-score=0.78) DNA repair	RS3_MYCMS (100.00%)	5 Equivalog	Essential
AMW76615 (MMSYN1_0666) L22: ribosomal protein L22	AVX54915 (JCVISYN3A_0666) rplV: 50S ribosomal protein L22	GO:0042255 (C-score=0.51) ribosome assembly	RL22_MYCMS (99.10%)	5 Equivalog	Essential
AMW76616 (MMSYN1_0667) S19: ribosomal protein S19	AVX54916 (JCVISYN3A_0667) rpsS: 30S ribosomal protein S19	GO:0000028 (C-score=0.98) ribosomal small subunit assembly	RS19_MYCMS (100.00%)	5 Equivalog	Essential
AMW76617 (MMSYN1_0668) L2: ribosomal protein L2	AVX54917 (JCVISYN3A_0668) rplB: 50S ribosomal protein L2	GO:0002181 (C-score=0.98) cytoplasmic translation	RL2_MYCMS (99.65%)	5 Equivalog	Essential
AMW76618 (MMSYN1_0669) L23: ribosomal protein L23	AVX54918 (JCVISYN3A_0669) rplW: 50S ribosomal protein L23	GO:0000027 (C-score=0.90) ribosomal large subunit assembly	RL23_MYCMS (98.94%)	4 Probable	Essential
AMW76619 (MMSYN1_0670) L4: 50S ribosomal protein L4	AVX54919 (JCVISYN3A_0670) rplD: 50S ribosomal protein L4	GO:0006412 (C-score=0.98) translation	RL4_MYCMS (99.52%)	5 Equivalog	Essential
AMW76620 (MMSYN1_0671) L3: 50S ribosomal protein L3	AVX54920 (JCVISYN3A_0671) rplC: 50S ribosomal protein L3	GO:0000027 (C-score=0.58) ribosomal large subunit assembly	RL3_MYCMS (99.10%)	5 Equivalog	Essential
AMW76621 (MMSYN1_0672) S10: ribosomal protein S10	AVX54921 (JCVISYN3A_0672) rpsJ: 30S ribosomal protein S10	GO:0006412 (C-score=0.84) translation	RS10_MYCMS (99.02%)	5 Equivalog	Essential
AMW76622 (MMSYN1_0684) FolD: Tetrahydrofolate interconversion	AVX54922 (JCVISYN3A_0684) folD: Bifunctional 5,10 -methylene-tetrahydrofolate dehydrogenase/5,10-methylene- tetrahydrofolate cyclohydrolase	GO:0004487 (C-score=0.52) methylenetetrahydrofolate dehydrogenase (NAD+) activity	FOLD_MYCMS (96.53%)	4 Probable	Quasi essential
AMW76623 (MMSYN1_0685) KtrAB: Potassium uptake protein	AVX54923 (JCVISYN3A_0685) natA: Sodium transporter	GO:0071805 (C-score=0.51) potassium ion transmembrane transport	Q6MSL2_MYCMS (97.39%)	3 Putative	Essential
AMW76624 (MMSYN1_0686) TrkA: Potassium uptake protein	AVX54924 (JCVISYN3A_0686) trkA: Potassium transporter	GO:0015079 (C-score=0.59) potassium ion transmembrane transporter activity	C7LL21_MYCML (100.00%)	3 Putative	Essential
AMW76625 (MMSYN1_0687) GatB: aspartyl/glutamyl- tRNA(Asn/Gln)	AVX54925 (JCVISYN3A_0687) gatB: Glutamyl-tRNA amidotransferase subunit B	GO:0070681 (C-score=1.00) glutaminyl-tRNAGln biosynthesis via transamidation	GATB_MYCMS (99.16%)	5 Equivalog	Essential
AMW76626 (MMSYN1_0688) GatA: aspartyl/glutamyl- tRNA(Asn/Gln)	AVX54926 (JCVISYN3A_0688) gatA: Glutamyl-tRNA amidotransferase subunit A	GO:0004040 (C-score=0.87) amidase activity	Q6MSL0_MYCMS (98.14%)	4 Probable	Essential
AMW76627 (MMSYN1_0689) GatC: aspartyl/glutamyl- tRNA(Asn/Gln)	AVX54927 (JCVISYN3A_0689) gatC: Glutamyl-tRNA amidotransferase subunit C	GO:0070681 (C-score=0.60) glutaminyl-tRNAGln biosynthesis via transamidation	Q6MSK9_MYCMS (98.98%)	5 Equivalog	Essential
AMW76628 (MMSYN1_0690) DnlJ: DNA ligase, NAD- dependent	AVX54928 (JCVISYN3A_0690) ligA: DNA ligase (NAD(+))	GO:0003911 (C-score=0.88) DNA ligase (NAD+) activity	DNLJ_MYCMS (97.60%)	5 Equivalog	Essential
AMW76629 (MMSYN1_0691) membrane protein, putative	AVX54929 (JCVISYN3A_0691) Uncharacterized protein	GO:0015893 (C-score=0.61) drug transport	Q6MSK7_MYCMS (97.62%)	2 Generic	Essential
AMW76630 (MMSYN1_0692) pseudouridine synthase, RluA family	AVX54930 (JCVISYN3A_0692) Uncharacterized pseudouridine synthase	GO:0000455 (C-score=0.59) enzyme-directed rRNA pseudouridine synthesis	Q6MSK6_MYCMS (97.02%)	2 Generic	Non essential
AMW76631 (MMSYN1_0693) CAAX protease	AVX54931 (JCVISYN3A_0693) Uncharacterized protease	GO:0005216 (C-score=0.56) ion channel activity	Q6MSK5_MYCMS (94.10%)	2 Generic	Essential
AMW76632 (MMSYN1_0694) PtsH: Phosphocarrier protein HPr	AVX54932 (JCVISYN3A_0694) ptsH: Phosphocarrier protein	GO:0008982 (C-score=0.56) protein-N(PI )-phosphohistidine-sugar phosphotransferase activity	Q6MSK4_MYCMS (96.63%)	4 Probable	Essential
AMW76633 (MMSYN1_0695) PcrA: ATP-dependent DNA helicase	AVX54933 (JCVISYN3A_0695) pcrA: ATP-dependent DNA helicase	GO:0060543 (C-score=0.73) negative regulation of strand invasion	Q6MSK3_MYCMS (96.40%)	4 Probable	Quasi essential
AMW76634 (MMSYN1_0696) RDD family protein	AVX54934 (JCVISYN3A_0696) Uncharacterized transporter	GO:0044119 (C-score=0.92) growth of symbiont in host cell	Q6MSK2_MYCMS (94.02%)	1 Unknown	Essential
AMW76635 (MMSYN1_0697) glycosyltransferase, group 2 family protein	AVX54935 (JCVISYN3A_0697) Uncharacterized glycosyl transferase	GO:0019348 (C-score=0.57) dolichol metabolic process	Q6MSK1_MYCMS (97.80%)	2 Generic	Quasi essential
AMW76636 (MMSYN1_0706) ABC transporter, permease protein	AVX54936 (JCVISYN3A_0706) thiB: Thiamine ABC transporter permease	GO:0022804 (C-score=0.67) active transmembrane transporter activity	Q6MSI6_MYCMS (95.13%)	3 Putative	Quasi essential
AMW76637 (MMSYN1_0707) ABC transporter, ATP-binding protein	AVX54937 (JCVISYN3A_0707) thiQ: Thiamine ABC transporter ATP-binding protein	GO:0015424 (C-score=0.61) amino acid-transporting ATPase activity	Q6MSH1_MYCMS (98.39%)	3 Putative	Quasi essential
AMW76638 (MMSYN1_0708) high affinity transport system protein p37	AVX54938 (JCVISYN3A_0708) thiB: Thiamine ABC transporter substrate-binding protein	GO:0016023 (C-score=0.56) cytoplasmic, membrane-bounded vesicle	Q6MSI4_MYCMS (82.49%)	3 Putative	Quasi essential
AMW76639 (MMSYN1_0710) Cof-like hydrolase	AVX54939 (JCVISYN3A_0710) Uncharacterized hydrolase	GO:0016311 (C-score=0.79) dephosphorylation	Q6MSG9_MYCMS (95.82%)	2 Generic	Quasi essential
AMW76640 (MMSYN1_0726) NagB: glucosamine-6-phosphate deaminase	AVX54940 (JCVISYN3A_0726) nagB: Glucosamine-6-phosphate deaminase	GO:0006043 (C-score=0.84) glucosamine catabolic process	C7LL51_MYCML (99.59%)	5 Equivalog	Non essential
AMW76641 (MMSYN1_0727) TpiA: triose-phosphate isomerase	AVX54941 (JCVISYN3A_0727) tpiA: Triose-phosphate isomerase	GO:0046166 (C-score=0.98) glyceraldehyde-3-phosphate biosynthetic process	C7LL52_MYCML (99.19%)	5 Equivalog	Essential
AMW76642 (MMSYN1_0728) HAD hydrolase, family IIB	AVX54942 (JCVISYN3A_0728) Uncharacterized hydrolase	GO:0016311 (C-score=0.68) dephosphorylation	C7LL53_MYCML (100.00%)	2 Generic	Non essential
AMW76643 (MMSYN1_0729) pgm_bpd_ind: phosphoglycerate mutase	AVX54943 (JCVISYN3A_0729) pgm: Phosphoglycerate mutase (2,3-diphosphoglycerate- independent)	GO:0046537 (C-score=0.89) 2,3-bisphosphoglycerate- independent phosphoglycerate mutase activity	C7LL54_MYCML (100.00%)	5 Equivalog	Essential
AMW76644 (MMSYN1_0730) hypothetical protein	AVX54944 (JCVISYN3A_0730) Uncharacterized protein	GO:0016853 (C-score=0.59) isomerase activity	C7LL55_MYCML (100.00%)	1 Unknown	Quasi essential
AMW76645 (MMSYN1_0732) DeoC: deoxyribose-phosphate aldolase	AVX54945 (JCVISYN3A_0732) deoC: Deoxyribose-phosphate aldolase	GO:0004139 (C-score=1.00) deoxyribose-phosphate aldolase activity	DEOC_MYCMS (82.78%)	3 Putative	Non essential
AMW76646 (MMSYN1_0733) PgcA: Phosphoglucomutase	AVX54946 (JCVISYN3A_0733) deoB: Phosphopentomutase	GO:0004614 (C-score=0.89) phosphoglucomutase activity	Q6MSE6_MYCMS (98.21%)	4 Probable	Essential
AMW76647 (MMSYN1_0747) PunA: Purine nucleoside phosphorylase	AVX54947 (JCVISYN3A_0747) punA: Purine-nucleoside phosphorylase	GO:0004731 (C-score=0.97) purine-nucleoside phosphorylase activity	C7LL69_MYCML (100.00%)	4 Probable	Quasi essential
AMW76648 (MMSYN1_0771) NrdE_NrdA: ribonucleoside- diphosphate reductase,	AVX54948 (JCVISYN3A_0771) nrdE: Ribonucleotide- diphosphate reductase subunit alpha	GO:0004748 (C-score=1.00) ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	Q6MSC5_MYCMS (97.16%)	5 Equivalog	Quasi essential
AMW76649 (MMSYN1_0772) NrdI: ribonucleotide reductase related	AVX54949 (JCVISYN3A_0772) nrdI: Ribonucleotide reductase assembly protein	GO:0010181 (C-score=0.90) FMN binding	NRDI_MYCMS (98.09%)	5 Equivalog	Essential
AMW76650 (MMSYN1_0773) NrdF: Ribonucleoside- diphosphate reductase	AVX54950 (JCVISYN3A_0773) nrdF: Ribonucleoside- diphosphate reductase subunit beta	GO:0004748 (C-score=0.99) ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	Q6MSC3_MYCMS (99.71%)	4 Probable	Quasi essential
AMW76651 (MMSYN1_0774) SecG: preprotein translocase	AVX54951 (JCVISYN3A_0774) secG: Preprotein translocase subunit	GO:0015031 (C-score=0.83) protein transport	C7LL95_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76652 (MMSYN1_0775) RNase_R: ribonuclease R	AVX54952 (JCVISYN3A_0775) rnr: Ribonuclease R	GO:0000175 (C-score=0.98) 3'-5'-exoribonuclease activity	C7LL96_MYCML (100.00%)	5 Equivalog	Essential
AMW76653 (MMSYN1_0776) SmpB: SsrA-binding protein	AVX54953 (JCVISYN3A_0776) smpB: SsrA-binding protein	GO:0070930 (C-score=1.00) trans-translation-dependent protein tagging	C7LL97_MYCML (100.00%)	5 Equivalog	Essential
AMW76654 (MMSYN1_0777) hypothetical protein	AVX54954 (JCVISYN3A_0777) Uncharacterized protein	GO:0003723 (C-score=0.51) RNA binding	C7LL98_MYCML (100.00%)	1 Unknown	Quasi essential
AMW76655 (MMSYN1_0778) hypothetical protein	AVX54955 (JCVISYN3A_0778) Uncharacterized protein	GO:0090563 (C-score=0.63) protein-phosphocysteine-sugar phosphotransferase activity	C7LL99_MYCML (100.00%)	1 Unknown	Essential
AMW76656 (MMSYN1_0779) PtsG: PTS system glucose- specific EIICB	AVX54956 (JCVISYN3A_0779) ptsG: PTS sugar transporter	GO:0090563 (C-score=0.87) protein-phosphocysteine-sugar phosphotransferase activity	C7LLA0_MYCML (100.00%)	4 Probable	Essential
AMW76657 (MMSYN1_0787) ATPase-IIIB_Mg: magnesium- translocating P-type	AVX54957 (JCVISYN3A_0787) mgtA: Magnesium-translocating P-type ATPase	GO:0015662 (C-score=0.78) ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism	Q6MSB0_MYCMS (98.73%)	5 Equivalog	Essential
AMW76658 (MMSYN1_0789) AtpC: ATP synthase epsilon chain	AVX54958 (JCVISYN3A_0789) atpC: F0F1 ATP synthase subunit delta/epsilon	GO:0046933 (C-score=1.00) proton-transporting ATP synthase activity, rotational mechanism	Q6MS95_MYCMS (97.98%)	4 Probable	Essential
AMW76659 (MMSYN1_0790) AtpD: ATP synthase subunit beta	AVX54959 (JCVISYN3A_0790) atpD: F0F1 ATP synthase subunit beta	GO:0046933 (C-score=0.75) proton-transporting ATP synthase activity, rotational mechanism	ATPB_MYCMS (99.16%)	4 Probable	Essential
AMW76660 (MMSYN1_0791) ATPsyn_F1gamma: ATP synthase F1, gamma subunit	AVX54960 (JCVISYN3A_0791) atpG: F0F1 ATP synthase subunit gamma	GO:0042777 (C-score=0.60) plasma membrane ATP synthesis coupled proton transport	ATPG_MYCMS (97.86%)	5 Equivalog	Essential
AMW76661 (MMSYN1_0792) atpA: ATP synthase F1, alpha subunit	AVX54961 (JCVISYN3A_0792) atpA: F0F1 ATP synthase subunit alpha	GO:0043531 (C-score=0.71) ADP binding	ATPA_MYCMS (97.14%)	5 Equivalog	Essential
AMW76662 (MMSYN1_0793) ATP_synt_delta: ATP synthase F1, delta subunit	AVX54962 (JCVISYN3A_0793) atpD: F0F1 ATP synthase subunit delta	GO:0015986 (C-score=0.70) ATP synthesis coupled proton transport	ATPD_MYCMS (96.13%)	5 Equivalog	Essential
AMW76663 (MMSYN1_0794) ATP_synt_b: ATP synthase F0, B subunit	AVX54963 (JCVISYN3A_0794) atpF: F0F1 ATP synthase subunit B	GO:0046933 (C-score=0.70) proton-transporting ATP synthase activity, rotational mechanism	ATPF_MYCMS (97.24%)	5 Equivalog	Essential
AMW76664 (MMSYN1_0795) ATP_synt_c: ATP synthase F0, C subunit	AVX54964 (JCVISYN3A_0795) atpE: F0F1 ATP synthase subunit C	GO:0015318 (C-score=0.53) inorganic solute uptake transmembrane transporter activity	ATPL_MYCMS (97.03%)	5 Equivalog	Essential
AMW76665 (MMSYN1_0796) AtpB: ATP synthase subunit a	AVX54965 (JCVISYN3A_0796) atpB: F0F1 ATP synthase subunit A	GO:0046933 (C-score=0.78) proton-transporting ATP synthase activity, rotational mechanism	Q6MS88_MYCMS (98.61%)	4 Probable	Essential
AMW76666 (MMSYN1_0797) hypothetical protein	AVX54966 (JCVISYN3A_0797) Uncharacterized protein	GO:0005737 (C-score=0.91) cytoplasm	Q6MS87_MYCMS (68.63%)	1 Unknown	Essential
AMW76667 (MMSYN1_0798) upp: uracil phosphoribosyltransferase	AVX54967 (JCVISYN3A_0798) upp: Uracil phosphoribosyltransferase	GO:0006223 (C-score=0.85) uracil salvage	UPP_MYCMS (99.03%)	5 Equivalog	Essential
AMW76668 (MMSYN1_0799) glyA, transferase	AVX54968 (JCVISYN3A_0799) glyA: Serine hydroxymethyltransferase	GO:1904482 (C-score=0.64) cellular response to tetrahydrofolate	GLYA_MYCMS (96.61%)	3 Putative	Quasi essential
AMW76669 (MMSYN1_0800) RpiB: ribose 5-phosphate isomerase B	AVX54969 (JCVISYN3A_0800) rpiB: Ribose 5-phosphate isomerase B	GO:0004751 (C-score=0.98) ribose-5-phosphate isomerase activity	Q6MS84_MYCMS (92.95%)	5 Equivalog	Quasi essential
AMW76670 (MMSYN1_0803) RpoC: DNA-directed RNA polymerase, beta'	AVX54970 (JCVISYN3A_0803) rpoC: DNA-directed RNA polymerase subunit beta'	GO:0003968 (C-score=0.57) RNA-directed RNA polymerase activity	RPOC_MYCMS (97.61%)	5 Equivalog	Essential
AMW76671 (MMSYN1_0804) RpoB: DNA-directed RNA polymerase, beta subunit	AVX54971 (JCVISYN3A_0804) rpoB: DNA-directed RNA polymerase subunit beta	GO:0003899 (C-score=0.99) DNA-directed RNA polymerase activity	RPOB_MYCMS (98.30%)	5 Equivalog	Essential
AMW76672 (MMSYN1_0805) transcription factor	AVX54972 (JCVISYN3A_0805) Uncharacterized protein	GO:0018117 (C-score=0.92) protein adenylylation	C7LMJ6_MYCML (21.87%)	2 Generic	Essential
AMW76673 (MMSYN1_0806) L12: ribosomal protein L7/L12	AVX54973 (JCVISYN3A_0806) rplG: 50S ribosomal protein L7/L12	GO:0006412 (C-score=1.00) translation	RL7_MYCMS (100.00%)	5 Equivalog	Essential
AMW76674 (MMSYN1_0807) L10: ribosomal protein L10	AVX54974 (JCVISYN3A_0807) rplJ: 50S ribosomal protein L10	GO:0070180 (C-score=0.65) large ribosomal subunit rRNA binding	RL10_MYCMS (97.58%)	4 Probable	Essential
AMW76675 (MMSYN1_0809) L1: ribosomal protein L1	AVX54975 (JCVISYN3A_0809) rplA: 50S ribosomal protein L1	GO:0000049 (C-score=0.62) tRNA binding	RL1_MYCMS (98.23%)	5 Equivalog	Quasi essential
AMW76676 (MMSYN1_0810) L11: ribosomal protein L11	AVX54976 (JCVISYN3A_0810) rplK: 50S ribosomal protein L11	GO:0000027 (C-score=0.99) ribosomal large subunit assembly	RL11_MYCMS (100.00%)	5 Equivalog	Quasi essential
AMW76677 (MMSYN1_0813) GalE: UDP-glucose 4-epimerase	AVX54977 (JCVISYN3A_0813) galE: UDP-glucose 4-epimerase GalE	GO:0003978 (C-score=0.93) UDP-glucose 4-epimerase activity	C7LLD2_MYCML (100.00%)	5 Equivalog	Essential
AMW76678 (MMSYN1_0814) glf: UDP-GALP_mutase: UDP- galactopyranose	AVX54978 (JCVISYN3A_0814) glf: UDP-galactopyranose mutase	GO:0008767 (C-score=0.92) UDP-galactopyranose mutase activity	C7LLD3_MYCML (100.00%)	5 Equivalog	Essential
AMW76679 (MMSYN1_0817) transcription factor, WhiA like	AVX54979 (JCVISYN3A_0817) whiA: Uncharacterized DNA- binding protein	GO:0043937 (C-score=1.00) regulation of sporulation	Q6MS46_MYCMS (90.61%)	2 Generic	Quasi essential
AMW76680 (MMSYN1_0818) lgt: Prolipoprotein diacylglyceryl transferase	AVX54980 (JCVISYN3A_0818) lgt: Diacylglyceryl transferase	GO:0008961 (C-score=1.00) phosphatidylglycerol- prolipoprotein diacylglyceryl transferase activity	Q6MS45_MYCMS (51.86%)	3 Putative	Essential
AMW76681 (MMSYN1_0819) TRX_reduct: thioredoxin- disulfide reductase	AVX54981 (JCVISYN3A_0819) trx: Thioredoxin-disulfide reductase	GO:0004791 (C-score=0.97) thioredoxin-disulfide reductase activity	Q6MS43_MYCMS (97.10%)	5 Equivalog	Essential
AMW76682 (MMSYN1_0820) lgt: Prolipoprotein diacylglyceryl transferase	AVX54982 (JCVISYN3A_0820) lgt: Diacylglyceryl transferase	GO:0008961 (C-score=0.85) phosphatidylglycerol- prolipoprotein diacylglyceryl transferase activity	Q6MS42_MYCMS (92.59%)	3 Putative	Essential
AMW76683 (MMSYN1_0821) hpr-ser: HPr(Ser) kinase/phosphatase	AVX54983 (JCVISYN3A_0821) hpr;ptsH: HPr(Ser) kinase/phosphatase	GO:0000155 (C-score=0.73) phosphorelay sensor kinase activity	HPRK_MYCMS (95.85%)	5 Equivalog	Non essential
AMW76684 (MMSYN1_0822) EcfS: Energy coupling-factor transporter,	AVX54984 (JCVISYN3A_0822) ecfS: Uncharacterized ECF transporter S component	GO:1901474 (C-score=0.55) azole transmembrane transporter activity	Q6MS41_MYCMS (85.71%)	2 Generic	Quasi essential
AMW76685 (MMSYN1_0823) FolC: folates to polyglutamate derivatives	AVX54985 (JCVISYN3A_0823) folC: Dihydrofolate synthase	GO:0006761 (C-score=0.58) dihydrofolate biosynthetic process	Q6MS40_MYCMS (88.38%)	4 Probable	Quasi essential
AMW76686 (MMSYN1_0824) UvrA: excinuclease ABC subunit A	AVX54986 (JCVISYN3A_0824) uvrA: Excinuclease ABC subunit A	GO:0035639 (C-score=0.50) purine ribonucleoside triphosphate binding	Q6MS39_MYCMS (98.73%)	5 Equivalog	Quasi essential
AMW76687 (MMSYN1_0825) UvrB: excinuclease ABC subunit B	AVX54987 (JCVISYN3A_0825) uvrB: Excinuclease ABC subunit B	GO:0009381 (C-score=0.76) excinuclease ABC activity	UVRB_MYCMS (98.95%)	5 Equivalog	Quasi essential
AMW76688 (MMSYN1_0826) YqeN: DNA polymerase clamp loader subunit	AVX54988 (JCVISYN3A_0826) yqeN: DNA polymerase III subunit delta	GO:0003689 (C-score=0.82) DNA clamp loader activity	C7LLE5_MYCML (100.00%)	4 Probable	Essential
AMW76689 (MMSYN1_0827) hypothetical protein	AVX54989 (JCVISYN3A_0827) Uncharacterized protein	GO:0000055 (C-score=0.53) ribosomal large subunit export from nucleus	C7LLE6_MYCML (100.00%)	1 Unknown	Quasi essential
AMW76690 (MMSYN1_0830) hypothetical protein	AVX54990 (JCVISYN3A_0830) Uncharacterized protein	GO:0044769 (C-score=0.59) ATPase activity, coupled to transmembrane movement of ions, rotational mechanism	C7LLE8_MYCML (100.00%)	1 Unknown	Non essential
AMW76691 (MMSYN1_0831) prs: Ribose-phosphate pyrophosphokinase	AVX54991 (JCVISYN3A_0831) prs: Phosphoribosylpyrophosphate synthetase	GO:0006015 (C-score=0.71) 5-phosphoribose 1-diphosphate biosynthetic process	C7LLE9_MYCML (100.00%)	4 Probable	Essential
AMW76692 (MMSYN1_0832) pth: aminoacyl-tRNA hydrolase	AVX54992 (JCVISYN3A_0832) pth: Aminoacyl-tRNA hydrolase	GO:0004045 (C-score=1.00) aminoacyl-tRNA hydrolase activity	C7LLF0_MYCML (100.00%)	5 Equivalog	Essential
AMW76693 (MMSYN1_0833) L9: ribosomal protein L9	AVX54993 (JCVISYN3A_0833) rplI: 50S ribosomal protein L9	GO:0019843 (C-score=0.62) rRNA binding	C7LLF1_MYCML (100.00%)	5 Equivalog	Quasi essential
AMW76694 (MMSYN1_0834) DnaC: Replicative DNA helicase	AVX54994 (JCVISYN3A_0834) dnaC: Replicative DNA helicase	GO:0006268 (C-score=0.99) DNA unwinding involved in DNA replication	C7LLF2_MYCML (100.00%)	5 Equivalog	Essential
AMW76695 (MMSYN1_0835) lipoprotein, putative	AVX54995 (JCVISYN3A_0835) Uncharacterized lipoprotein	GO:0005623 (C-score=0.50) cell	C7LLF3_MYCML (100.00%)	1 Unknown	Essential
AMW76696 (MMSYN1_0836) EcfS: Energy coupling-factor transporter,	AVX54996 (JCVISYN3A_0836) ecfS: Uncharacterized ECF transporter S component	GO:0000155 (C-score=0.92) phosphorelay sensor kinase activity	C7LLF4_MYCML (100.00%)	2 Generic	Essential
AMW76697 (MMSYN1_0837) CysRS: Cysteinyl-tRNA synthetase	AVX54997 (JCVISYN3A_0837) cysRS: Cysteine--tRNA ligase	GO:0006423 (C-score=1.00) cysteinyl-tRNA aminoacylation	C7LLF5_MYCML (100.00%)	5 Equivalog	Essential
AMW76698 (MMSYN1_0838) RNA methyltransferase, TrmH family, group 3	AVX54998 (JCVISYN3A_0838) rlmB: 23S rRNA (guanosine(2251 )-2'-O)-methyltransferase	GO:0070039 (C-score=0.71) rRNA (guanosine-2'-O-)-methylt ransferase activity	C7LLF6_MYCML (100.00%)	2 Generic	Non essential
AMW76699 (MMSYN1_0839) SecE: preprotein translocase, SecE subunit	AVX55000 (JCVISYN3A_0839) secE: Preprotein translocase subunit	GO:0009306 (C-score=0.85) protein secretion	C7LLF7_MYCML (100.00%)	5 Equivalog	Essential
AMW76700 (MMSYN1_0840) NusG: Transcription termination/antitermination	AVX55001 (JCVISYN3A_0840) nusG: Antitermination protein	GO:0032784 (C-score=0.68) regulation of DNA-templated transcription, elongation	C7LLF8_MYCML (100.00%)	4 Probable	Quasi essential
AMW76701 (MMSYN1_0851) lipoprotein, putative	AVX55002 (JCVISYN3A_0851) Uncharacterized lipoprotein	GO:0043231 (C-score=0.71) intracellular membrane-bounded organelle	Q6MTS8_MYCMS (32.22%)	1 Unknown	Non essential
AMW76702 (MMSYN1_0852) hypothetical protein	AVX55003 (JCVISYN3A_0852) Uncharacterized protein	GO:0048037 (C-score=0.58) cofactor binding	Q6MU18_MYCMS (11.82%)	1 Unknown	Non essential
AMW76703 (MMSYN1_0853) hypothetical protein	AVX55004 (JCVISYN3A_0853) Uncharacterized protein	GO:0090563 (C-score=0.91) protein-phosphocysteine-sugar phosphotransferase activity	Q6MTK5_MYCMS (15.82%)	1 Unknown	Non essential
AMW76704 (MMSYN1_0859) TopA: DNA topoisomerase 1	AVX55005 (JCVISYN3A_0859) topA: DNA topoisomerase I	GO:0003917 (C-score=0.62) DNA topoisomerase type I activity	C7LLH7_MYCML (100.00%)	4 Probable	Essential
AMW76705 (MMSYN1_0870) Putative C4-dicarboxylate anaerobic carrier	AVX55006 (JCVISYN3A_0870) Uncharacterized C4-dicarboxylate ABC transporter	GO:0015318 (C-score=0.53) inorganic solute uptake transmembrane transporter activity	Q6MS63_MYCMS (98.64%)	2 Generic	Quasi essential
AMW76706 (MMSYN1_0872) GTP-binding protein YchF like	AVX55007 (JCVISYN3A_0872) ychF: Uncharacterized ATPase	GO:0043023 (C-score=0.79) ribosomal large subunit binding	Q6MS65_MYCMS (97.53%)	2 Generic	Quasi essential
AMW76707 (MMSYN1_0873) PF06107 family protein	AVX55008 (JCVISYN3A_0873) Uncharacterized protein	GO:0044260 (C-score=0.61) cellular macromolecule metabolic process	Q6MS66_MYCMS (98.48%)	1 Unknown	Quasi essential
AMW76708 (MMSYN1_0874) RsmG: Ribosomal RNA small subunit	AVX55009 (JCVISYN3A_0874) rsmG: 16S rRNA (guanine(527)-N (7))-methyltransferase	GO:0070043 (C-score=0.82) rRNA (guanine-N7-)-methyltrans ferase activity	RSMG_MYCMS (69.56%)	5 Equivalog	Non essential
AMW76709 (MMSYN1_0875) PgsA: CDP-diacylglycerol-- glycerol-3-phosphate	AVX55010 (JCVISYN3A_0875) pgsA: CDP-diacylglycerol-- glycerol-3-phosphate 3-phosphatidyltransferase	GO:0017169 (C-score=0.58) CDP-alcohol phosphatidyltransferase activity	Q6MS68_MYCMS (97.03%)	5 Equivalog	Essential
AMW76710 (MMSYN1_0876) amino acid permease	AVX55011 (JCVISYN3A_0876) Uncharacterized amino acid permease	GO:1990822 (C-score=0.50) basic amino acid transmembrane transport	Q6MS69_MYCMS (97.34%)	2 Generic	Non essential
AMW76711 (MMSYN1_0877) membrane protein, putative	AVX55012 (JCVISYN3A_0877) Uncharacterized protein	GO:0015318 (C-score=0.58) inorganic solute uptake transmembrane transporter activity	Q6MS70_MYCMS (96.46%)	2 Generic	Essential
AMW76712 (MMSYN1_0878) amino acid permease	AVX55013 (JCVISYN3A_0878) Uncharacterized amino acid permease	GO:0015370 (C-score=0.50) solute:sodium symporter activity	Q6MS71_MYCMS (96.14%)	2 Generic	Quasi essential
AMW76713 (MMSYN1_0879) putative magnesium-importing ATPase	AVX55014 (JCVISYN3A_0879) corA: Magnesium transporter	GO:0005391 (C-score=0.50) sodium:potassium-exchanging ATPase activity	Q6MS72_MYCMS (93.26%)	2 Generic	Essential
AMW76714 (MMSYN1_0881) membrane protein, putative	AVX55015 (JCVISYN3A_0881) Uncharacterized MFS transporter	GO:0098656 (C-score=0.52) anion transmembrane transport	Q6MS76_MYCMS (92.05%)	2 Generic	Essential
AMW76715 (MMSYN1_0885) GidA: tRNA uridine 5-carboxymethylaminomethyl	AVX55016 (JCVISYN3A_0885) mnmG;gidA: tRNA uridine(34) 5-carboxymethylaminomethyl synthesis enzyme	GO:0002098 (C-score=1.00) tRNA wobble uridine modification	MNMG_MYCMS (98.09%)	5 Equivalog	Quasi essential
AMW76716 (MMSYN1_0886) GltP: Proton glutamate symport protein	AVX55017 (JCVISYN3A_0886) gltP: Proton-glutamate symporter	GO:0015813 (C-score=0.58) L-glutamate transport	Q6MRW7_MYCMS (96.47%)	3 Putative	Quasi essential
AMW76717 (MMSYN1_0887) pyridine nucleotide-disulfide oxidoreductase	AVX55018 (JCVISYN3A_0887) cdr: Coenzyme A Disulfide Reductase	GO:0050451 (C-score=0.70) CoA-disulfide reductase activity	Q6MRW6_MYCMS (96.08%)	3 Putative	Quasi essential
AMW76718 (MMSYN1_0906) Putative choline/ethanolamine kinase	AVX55019 (JCVISYN3A_0906) Uncharacterized protein	GO:0000166 (C-score=0.60) nucleotide binding	Q6MRS6_MYCMS (87.14%)	2 Generic	Essential
AMW76719 (MMSYN1_0907) Cof-like hydrolase	AVX55020 (JCVISYN3A_0907) Uncharacterized hydrolase	GO:0016311 (C-score=0.83) dephosphorylation	Q6MRS5_MYCMS (95.08%)	2 Generic	Quasi essential
AMW76720 (MMSYN1_0908) YidC: Membrane protein insertase	AVX55021 (JCVISYN3A_0908) yidC: Membrane protein insertase	GO:0008565 (C-score=0.74) protein transporter activity	Q6MRS4_MYCMS (99.75%)	4 Probable	Essential
AMW76721 (MMSYN1_0909) RnpA: ribonuclease P protein component	AVX55022 (JCVISYN3A_0909) rnpA: Ribonuclease P protein component	GO:0001682 (C-score=0.87) tRNA 5'-leader removal	RNPA_MYCMS (96.33%)	5 Equivalog	Essential
AMW76722 (MMSYN1_0910) L34: ribosomal protein L34	AVX55023 (JCVISYN3A_0910) rpmH: 50S ribosomal protein L34	GO:0006412 (C-score=0.99) translation	RL34_MYCMS (97.73%)	5 Equivalog	Quasi essential

(a) The one-sentence function summary in the "Our prediction" column is generated using the most specific high confidence predicted GO term, which is selected by the following procedures. Firstly, for each GO term, we calculate its background probability, which equals to the number of UniProt proteins annotated to that GO term divied by total number of UniProt proteins. Secondly, for a target protein, all its COFACTOR predicted GO terms are ranked in ascending order of background probability. Finally, from the ranked list, we select the first GO term with C-score≥0.5; if unavailable, the first term with C-score≥0.4 is selected; if still unavailable, the first term with C-score≥0.3 is selected.
(b) "Essentiality" in the table was determined by transposon mutagenesis and flux-balance analysis, and does not strictly refer to whether the loss of the gene leads to inviable cells.

List of targets newly introduced in JCVI-syn3A and not present in JCVI-syn3.0

JCVI-syn3A accession and NCBI annotation	Our prediction (click to view)	Closest homolog in M. mycoides (sequence identity)	Essentiality
AVX54838.1 (JCVISYN3A_0522) Cell division protein	GO:0043093 (C-score=0.87) FtsZ-dependent cytokinesis	Q6MT25_MYCMS (98.57%)	Non essential
AVX54837.1 (JCVISYN3A_0521) Cell division protein	GO:0000917 (C-score=1.00) barrier septum assembly	Q6MT26_MYCMS (97.10%)	Non essential
AVX54836.1 (JCVISYN3A_0520) Uncharacterized hydrolase	GO:0006629 (C-score=0.65) lipid metabolic process	Q6MT27_MYCMS (99.09%)	Non essential
AVX54861.1 (JCVISYN3A_0592) Uncharacterized protein	GO:0016043 (C-score=0.52) cellular component organization	Q6MSW5_MYCMS (38.05%)	Quasi essential
JCVISYN3A_0546 (JCVISYN3A_0546) JCVISYN3A_0546	GO:0016311 (C-score=0.54) dephosphorylation	Q6MT02_MYCMS (41.82%)	Non essential
AVX54862.1 (JCVISYN3A_0593) Uncharacterized protein	GO:0016020 (C-score=0.58) membrane	Q6MSU4_MYCMS (30.05%)	Non essential
JCVISYN3A_0602 (JCVISYN3A_0602) JCVISYN3A_0602	GO:0016021 (C-score=0.83) integral component of membrane	C7LMY3_MYCML (25.34%)	Non essential
AVX54858.1 (JCVISYN3A_0548) tRNA (cytidine(34)-2'-O)-methy ltransferase	GO:0002131 (C-score=0.75) wobble position cytosine ribose methylation	Q6MT01_MYCMS (98.90%)	Non essential
AVX54850.1 (JCVISYN3A_0538) Uncharacterized protein	GO:0003746 (C-score=0.98) translation elongation factor activity	Q6MT09_MYCMS (98.51%)	Non essential
AVX54843.1 (JCVISYN3A_0527) Uncharacterized protein	GO:0006418 (C-score=0.54) tRNA aminoacylation for protein translation	Q6MT19_MYCMS (99.42%)	Non essential
AVX54859.1 (JCVISYN3A_0549) Non-canonical purine NTP pyrophosphatase	GO:0047429 (C-score=0.98) nucleoside-triphosphate diphosphatase activity	IXTPA_MYCMS (96.00%)	Non essential
AVX54872.1 (JCVISYN3A_0610) DNA-formamidopyrimidine glycosylase	GO:0008534 (C-score=0.94) oxidized purine nucleobase lesion DNA N-glycosylase activity	Q6MST7_MYCMS (98.18%)	Non essential
AVX54882.1 (JCVISYN3A_0622) Uncharacterized lipoprotein	GO:0005737 (C-score=0.67) cytoplasm	Q6MSS6_MYCMS (97.50%)	Non essential
AVX54867.1 (JCVISYN3A_0605) Uncharacterized protein	GO:0003824 (C-score=0.85) catalytic activity	C7LKV0_MYCML (100.00%)	Non essential
AVX54866.1 (JCVISYN3A_0604) Uncharacterized protein	GO:0005618 (C-score=0.91) cell wall	C7LKU9_MYCML (100.00%)	Non essential
AVX54883.1 (JCVISYN3A_0623) Uncharacterized protein	GO:0044424 (C-score=0.62) intracellular part	Q6MSS4_MYCMS (37.21%)	Non essential

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Reference:

• Chengxin Zhang, Wei Zheng, Micah Cheng, Peter L Freddolino, Yang Zhang (2020) "Functions of essential genes and a scale-free protein interaction network revealed by structure-based function and interaction prediction for a minimal genome" Journal of Proteome Research.