| (a) | Column 1: The rank of the the template as determined by the Z-score. The template is with the highest Zscore is ranked 1. |
| (b) | Column 2: Name of the template PDB from which the particular model was generates. Templates are ranked according to the Z-score of the alignment. |
| (c) | Column 3: The length of the threading alignment between the query sequence and the template. |
| (d) | Column 4: The coverage of the aligment in calculated by dividing the length of the alignment (previous column) by the total length of the query complex. |
| (e) | Column 5: Zscore, or statistical significance, of the alignment upon which the ranking of the templates is based. The Zscore used here is the mean Zscore of the alignment of chain 1 only, the alignment of chain 2 only and the alignment of the complex as a whole. For further reading about Z-score please click here. |
| (f) | Column 6: The sequence identity between the query sequence and the template sequence. |
| (g) | Column 7: The confidence of the threading alignment is based on the Zscore and is interpolated from the COTH benchmarking results. The confidence is considered High if the Zscore of the alignment is greater than 2.5 or Low otherwise. |
| (h) | Column 8: The threading alignment between the query sequence and the template. |
| (i) | Column 9: The models generated from COTH threading alone. These models are generated before the superposition of the MUSTER individual chain templates onto the dimer templates identified by COTH threading. |
| (j) | Column 10: The final models generated by COTH after superpostion of the MUSTER individual chain templates onto the dimer templates identified by COTH threading. |
